柱层析法分离Triton X—45中烷基酚聚氧乙烯醚

从非离子型表面活性剂Triton X-45中分离单一聚合度的氧乙烯基化合物,可以用柱层析法分离。淋洗剂为三氯甲烷、乙醚:氯仿(1:99) 和乙醚:氯仿(1:1.2)。在乙醚:氯仿(1:1.2)的洗脱液馏份内可以收集到单一聚合度的烷基酚聚氧乙烯醚(n=5)。氧乙烯基的聚合度用核磁共振光谱法及场解吸质谱法确定。用高效液相色谱测定分离物的纯度为97%以上。该方法的特点是仪器简单、操作方便、分离时间短。一次分离300mg的Triton X-45样品,可得到烷基酚聚氧乙烯醚(n=5)约50mg,为研究烷基酚聚氧乙烯醚的氧乙烯基聚合度分布提供实验室制备标样的简易方法。

Separation of Polyoxyethylene Alkylphenoxy Ether from Triton X-45 by Column Chromatography

Single oligomer of ethylene oxide is separated from nonionic surfactentTriton X-45 with silica gel column by using chloroform, ether: chloroform(1:99) and ether: cholroform (1:1.2) as eluates. Polyoxyethylene alkylphenoxyether can be obtained from eluate ether:chloroform (1:1.2). The degree ofpolymerization of ethoxylate group is determined by NMR spectrometry andfield desorption mass spectrometry. The purity of polyoxyethylene alkylphenoxyether determined by HPLC is more than 97%. This method is simple toperform and reguires a minimum of equipment and short test time. About 50mg of polyoxyethylene alkylphenoxy ether is obtained from about 300 mgof Triton X-45 in a single analysis. It offers a sample preparation method ofsingle ethoxymer standard of known E_o number to determine ethoxymerdistribution of polyoxyethylene alkyphenoxy ether.