加勒比松树皮中原花青素的提取工艺研究

系统研究了以加勒比松树皮为原料提取原花青素的方法及工艺条件。研究对比了常规溶剂浸提、超声波提取及微波辅助提取等方法对原花青素提取得率的影响。实验结果表明,溶剂浸提采用 70% 乙醇溶液时原花青素的得率最高。在3种提取方法中,超声波提取法和微波辅助提取法提取效果较优。前者能在短时间内取得较高的提取得率,而后者需进一步延长微波辅助作用后溶剂浸提的时间才能达到较好的提取效果。超声波提取法的适宜提取条件为: 提取温度 50℃,功率 100 W,料液比1:11,提取时间 1.5 h;微波辅助提取法的适宜提取条件为: 功率 200 W,微波处理时间 30 s,料液比1:11,提取温度 50℃,提取时间 6 h。在上述条件下原花青素得率分别为 7.47% 和 7.69%。

Study on the Extraction Technology of Procyanidins from Caribbean Pine Bark

The method and technological conditions for extraction of extract procyanidins from Caribbean pine bark were systema-tically investigated. Three different extraction methods, including conventional solvent extraction, ultrasonic wave extraction and microwave-assisted extraction were compared. The results indicated that the extraction yield of procyanidins was highest when 70% ethanol was used as solvent. Ultrasonic wave extraction and microwave-assisted extraction are better among the three extraction methods. Ultrasonic wave extraction can get a high yield in a short-time extraction process. However in microwave-assisted extraction the solvent extraction time after the effect of microwave should be prolonged to obtain a high extraction yield. For ultrasonic wave extraction, the optimum conditions were as follows: extraction temperature 50℃, ultrasonic wave power 100 W, the ratio of raw materials to ethanol 1:11, and extraction time 1.5 h. The optimum conditions of microwave-assisted extraction were microwave power 200 W, radiation time with microwave 30 s, the ratio of solid to liquid 1:11, extraction temperature 50℃, and extraction time 6 h. As the results, the yields of procyanidins from Caribbean pine bark were 7.47% and 7.69% respectively under above conditions.