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掺氮TiO_(2-x)N_x薄膜托槽的细胞相容性研究
引用本文:张颖杰,曹宝成,王育华,李娜,张旭.掺氮TiO_(2-x)N_x薄膜托槽的细胞相容性研究[J].材料导报,2012,26(12):70-74,96.
作者姓名:张颖杰  曹宝成  王育华  李娜  张旭
作者单位:1. 兰州大学口腔医学院,兰州,730000
2. 兰州大学物理科学与技术学院,兰州,730000
3. 西北民族大学口腔医学院,兰州,730030
基金项目:甘肃省国际科技合作基金,教育部春晖计划,中央高校基本科研业务费专项资金
摘    要:采用射频磁控溅射法在普通MBT直丝弓金属托槽表面制备了锐钛矿相掺氮TiO2-xNx薄膜,通过MTT比色法、细胞形态学观察、扫描电镜及荧光染色法评价薄膜托槽的细胞学性能。结果表明,掺氮TiO2-xNx薄膜托槽的细胞毒性为0级,与对照组相比,薄膜托槽组细胞的相对增殖率差异无统计学意义(P0.05);扫描电镜图像显示细胞在薄膜托槽表面贴壁生长良好;荧光显微镜观察显示粘附在材料表面的细胞其细胞核呈亮黄绿色荧光,细胞质呈绿色荧光,细胞生长良好。由此证明掺氮TiO2-xNx薄膜托槽具有良好的细胞相容性。

关 键 词:掺氮TiO-xNx薄膜  MBT直丝弓金属托槽  细胞相容性

Cytocompatibility of the Nitrogen-doped TiO2-xNx Films on Brackets
ZHANG Yingjie , CAO Baocheng , WANG Yuhua , LI Na , ZHANG Xu.Cytocompatibility of the Nitrogen-doped TiO2-xNx Films on Brackets[J].Materials Review,2012,26(12):70-74,96.
Authors:ZHANG Yingjie  CAO Baocheng  WANG Yuhua  LI Na  ZHANG Xu
Affiliation:1 (1 School of Stomatology,Lanzhou University,Lanzhou 730000;2 School of Physical Science and Technology,Lanzhou University,Lanzhou 730000;3 School of Stomatology,Northwest University for Nationalities,Lanzhou 730030)
Abstract:Nitrogen-doped TiO2-xNx films were produced on the MBT straight wire appliance by the method of radio frequency magnetron sputtering and the cytocomptibility were evaluated by means of MTT methods,microscope observation,SEM and fluorescent staining method.According to MTT assay,the level of cytotoxicity of nitrogen-doped TiO2-xNx films was grade 0,and the percentage of cells proliferation of the two groups showed no statistical difference(P>0.05).The SEM photo showed the L929 cells grew well on the surface of the films.The nuclei was bright yellow-green fluorescence,cytoplasm was green fluorescent on the surface of the films under fluorescence microscope.The films showed a good cell compatibility.
Keywords:nitrogen-doped TiO2-xNx films  MBT straight wire appliance  cytocomptibility
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