Influence of elaidate and erucate on heart mitochondria |
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Authors: | Chi Ming Lee Hsu Fred A Kummerow |
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Affiliation: | (1) The Burnsides Research Laboratory, University of Illinois, 61801 Urbana, IL |
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Abstract: | Male, weanling rats were fed, for up to six weeks, corn oil (CO), rapeseed oil (RSO), partially hydrogenated fat (HF), or
a mixture of partially hydrogenated fat and corn oil (HF+CO). The respiratory activity of their isolated heart mitochondria,
their hormone-sensitive lipase activity, and the fatty acid compositions of the phospholipids of the mitochondria were determined.
The results indicated that heart mitochondria isolated from rats which had been fed corn oil (CO) had a higher rate of oxygen
uptake, showed higher respiratory control ratios, higher ADP/0 ratios and a higher rate of ATP synthesis than the heart mitochondria
isolated from those fed rapeseed oil or hydrogenated fats. The oxygen uptake rates of the rat heart mitochondria isolated
from each dietary group of rats was in order: oleyl carnitine ≫ erucyl carnitine > elaidyl carnitine. The decreased capacity
to oxidize substrate by heart mitochondria which had been isolated from the hearts of rats fed rapeseed or hydrogenated soybean
oil as compared with those fed corn oil as a sole source of dietary fat seemed related to the mitochondria lipid composition.
The type of dietary fat fed had a pronounced influence on the mitochondrial fatty acid compositions of phosphatidylcholine,
phosphatidylethanolamine, and cardiolipin. The lipase activity of the RSO-fed group did not show any increment with either
epinephrine or supplemental ATP treatment. The substrate preference for lipase activity in myocardium was corn oil-triglycerides
> trierucin > trielaidin > tripalmitin. However, cardiac lipid accumulation did not seem related to lipase activity in the
myocardium.
Taken from a thesis submitted by Chi Ming Lee Hsu in partial fulfillment of the Ph.D. degree in Food Science, University of
Illinois at Urbana-Champaign. |
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