一种新型蛋白聚糖类生物絮凝剂的分离纯化及组成分析

从土壤中筛选获得了一株絮凝剂产生菌 ,经初步鉴定为诺卡氏菌 (Nocardiasp .) .考察了该菌株的基本生长代谢特性 ,其合成的絮凝剂约 80 %以上分泌至胞外培养基中 .利用有机溶剂沉淀结合柱层析法从Nocardiasp .CCTCCM 2 0 10 0 5的发酵液中提取到一种新型生物絮凝剂REA - 11.分析表明 ,REA - 11是一种以半乳糖醛酸为主要结构单元的酸性蛋白聚糖

PURIFICATION AND IDENTIFICATION OF NOVEL BIOFLOCCULANT

This paper mainly discussed a flocculant-producing bacteria isolated from soil and the flocculating substance secreted into the medium.Preliminary studies identified the bacteria CCTCC M201005 as Nocardia species.Its growth and metabolism were studied.More than 80% of the flocculating activity was detected in the culture broth.A new bioflocculant--REA-11 was purified by repeated ethanol precipitation,ion exchange and gel chromatography from the culture broth.Its molecular mass was estimated to be over 10 ⁵g·mol ^-1 by the gel filtration method.The results of investigation on the purified REA-11 and its acid hydrolysate by colcrimetric analysis,paper chromatography,HPLC and GC-MS suggested that REA-11 was a novel acidic polysaccharide mainly composed of galactocuronic acid with little acetic acid but no hexosamine detected.Protein was not a necessary part for the flocculating activity of REA-11,but mainly assisting in connecting the monosaccharides in the macromolecule or maintaining the dimensional conformation of the active biopolymer.