卵黄高磷蛋白磷酸肽-钙对受试小鼠钙表观吸收率的影响

本实验借助营养成分常规检测方法和原子吸收检测法对三种卵黄高磷蛋白磷酸肽-钙样品进行样品纯度检测和含钙量的分析。对样品采用羧甲基纤维素进行预处理后,选用葡萄糖酸钙市场上常见的补钙制剂与之比较,通过小鼠动物实验来研究这种补钙制剂对处于生长期的小鼠钙吸收率的影响。结果表明,在三种卵黄高磷蛋白磷酸肽-钙样品中,PPP3纯度最高且含钙量最高;在小鼠的体重增长过程中三种样品均没有显著差异,说明卵黄高磷蛋白磷酸肽-钙对小鼠的体重没有影响;在钙的表观吸收率方面,其中PPP3-钙高剂量组的钙的表观吸收率最高,这组数据表现为对空白组和对照组均有显著差异(p<0.05),往下依次为PPP3-钙中剂量组、PPP2-钙中剂量组、PPP1-钙中剂量组和PPP3-钙补剂低剂量组,均表现为与空白组差异显著(p<0.05)。

Effects of Phosvitin Phosphopeptides-calcium on Apparent Absorptivity of Calcium in Mice

Purity and calcium content of three phosvitin phosphopeptides-calcium samples were analyzed using the detection methods of routine ingredient analysis and atomic absorption. Comparison of sample pretreated using carboxymethyl cellulose with the calglucon as one kind of calcium-supplementing product in the market was conducted to determine the calcium-absorbing rate in mice during growing period. The results indicated that:(1) Both the purity and calcium-absorbing rate of PPP3-calcium sample are the highest one among the three samples;(2) There is no significant difference during growing period among the three samples;(3) The mice in the PPP3-calcium group with the highest calcium content have the highest calcium-absorbing rate,which shows that there are significant differences in comparison with the blank and control group(p<0.05). The order of the groups in the calcium-absorbing rate is as follows:the middle group of PPP3-calcium>middle group of PPP2-calcium>middle group of PPP1-calcium>low group of PPP3-calcium. All the groups show significant difference from the blank group.