| 壳寡糖季铵盐型基因载体制备的新方法研究 |
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| 引用本文： | 陈会英,赵轶男,丁雪,边庆龙,支德福,张树彪. 壳寡糖季铵盐型基因载体制备的新方法研究[J]. 化工新型材料, 2011, 0(Z2) |
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| 作者姓名： | 陈会英 赵轶男 丁雪 边庆龙 支德福 张树彪 |
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| 作者单位： | 大连民族学院生命科学学院生物化学工程国家民委教育部重点实验室; |
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| 基金项目： | 国家自然科学基金资助项目(20876027、21046008); 教育部新世纪优秀人才支持计划项目(NCET-08-0654); 中央高校基本科研业务费资助项目(DC10020103) |
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| 摘 要： | 首次以甲醇/水为溶剂合成壳寡糖季铵盐型基因载体,并采用1 H NMR表征技术对合成条件进行了研究。结果表明,反应溶剂、NaOH浓度、CH3I用量、反应时间、一步法/两步法合成步骤等都会影响壳寡糖季铵盐的合成。甲醇/水溶剂比NMP溶剂更有利于壳寡糖季铵盐产品的控制、减少副反应的发生;适量的NaOH浓度,有利于壳寡糖季铵盐的合成,NaOH浓度过低,不利于季铵盐的生成,NaOH浓度过高,易发生壳寡糖3,6位-OH的甲醚化副反应;足够量的CH3I和反应时间及两步法合成步骤有利于壳寡糖季铵盐的合成。本研究为具有潜在临床应用价值的壳寡糖季铵盐基因载体提供了一种低碳、绿色、经济、环境友好的合成新方法。
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| 关 键 词： | 壳寡糖 壳寡糖季铵盐 季铵化程度 1H-NMR |
Study on a new method of preparing trimethylated chitosan oligosaccharide for gene delivery |
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| Chen Huiying Zhao Yinan Ding Xue Bian Qinglong Zhi Defu Zhang Shubiao. Study on a new method of preparing trimethylated chitosan oligosaccharide for gene delivery[J]. New Chemical Materials, 2011, 0(Z2) |
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| Authors: | Chen Huiying Zhao Yinan Ding Xue Bian Qinglong Zhi Defu Zhang Shubiao |
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| Affiliation: | Chen Huiying Zhao Yinan Ding Xue Bian Qinglong Zhi Defu Zhang Shubiao (Key Laboratory of Bio-chemistry Engineering-The State Ethnic Affairs Commission-Ministry of Education,College of Life Science,Dalian Nationalities University,Dalian 116605) |
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| Abstract: | A new method for synthesizing N-trimethyl chitosan oligosaccharide was found by using methanol/water as solvent.1H NMR was employed in studying the influence of different synthesis conditions on the degree of quaternization of N-trimethyl chitosan.The study concluded that the synthesis conditions,including the reaction solvent,the concentration of NaOH,the amount of CH3I,the reaction time and the synthesis steps,could influence the synthesis of N-trimethyl chitosan oligosaccharide.Compared to NMP solvent,CH... |
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| Keywords: | chitosan oligosaccharide N-trimethyl chitosan oligosaccharide degree of quaternization 1H-NMR |
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