Water extractable phytochemicals from Capsicum pubescens (tree pepper) inhibit lipid peroxidation induced by different pro-oxidant agents in brain: in vitro |
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Authors: | G Oboh J B T Rocha |
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Affiliation: | (1) Biochemistry Department, Federal University of Technology, P.M.B. 704, Akure, Ondo State, Nigeria;(2) The Abdus Salam International Centre for Theoretical Physics, Strada Costiera 11, 34014 Trieste, Italy;(3) Departamento de Quimica, Universidade Federal de Santa Maria (UFSM), Campus Universitário – Camobi, 97105-900 Santa Maria RS, Brasil |
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Abstract: | This study seeks to determine the antioxidant and neuroprotective properties of aqueous extract of ripe and unripe Capsicum pubescens (tree pepper) on some pro-oxidant induced lipid peroxidation in rat’s brain (in vitro). The total phenol, vitamin C, ferric
reducing antioxidant property (FRAP) and Fe (II) chelating ability of the extracts of C. pubescens were determined. Thereafter, the ability of the extracts to inhibit lipid peroxidation (induced by FeSO4, sodium nitroprusside or quinolinic acid) in rat’s brain homogenates (in vitro) was determined. The results of the study
revealed that ripe C. pubescens had a significantly higher (P < 0.05) total phenol content ripe (113.7 mg/100 g), unripe (70.5 mg/100 g)] and reducing power than the unripe pepper. However,
there was no significant difference (P > 0.05) in the vitamin C ripe (231.5 μg/g), unripe (224.4 μg/g)] content and Fe (II) chelating ability of the extracts.
However, both extracts significantly (P < 0.05) inhibited lipid peroxidation induced by the pro-oxidant agents 25 μM Fe(II), 7 μM sodium nitroprusside and 1 mM
quinolinic acid] in the rat’s brain homogenates in a dose-dependent manner. Nevertheless, the ripe pepper extracts inhibited
MDA (Malondialdehyhide) production in the rat’s brain homogenates than the unripe pepper. Conversely, both extracts did not
significantly (P > 0.05) inhibit Fe (II)/H2O2 induced decomposition of deoxyribose. It was therefore concluded that ripe and unripe C. pubescens would inhibit lipid peroxidation in rat brain in vitro. However, the ripe pepper was a more potent inhibitor of lipid peroxidation
in the rat’s brain; this is probably due to its higher phenol content and reducing power. |
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Keywords: | Capsicum pubescens Phenol Brain Lipid peroxidation |
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