桦褐孔菌总黄酮的微波提取及美容功效研究

通过正交试验优化了桦褐孔菌总黄酮的提取工艺,测定了总黄酮提取液的酪氨酸酶抑制率,并制备了桦褐孔菌总黄酮雪花膏。进行了该雪花膏的性能测试和大白鼠皮肤抗氧化试验:包括用丙二醛(MDA)试剂盒测定MDA含量;用邻苯三酚自氧化法测定大白鼠超氧化物歧化酶(SOD)活性;活性氧(ROS)试剂盒体外测定皮肤组织羟自由基清除能力。得到了桦褐孔菌总黄酮的较优提取条件:乙醇体积分数为70%,料液比(g∶mL)为1∶40,微波辐射时间为120 s,功率为352 W,此条件下总黄酮的最高提取率为4.96%。质量浓度为0.024 g.L-1的桦褐孔菌总黄酮提取液加入量为7.5 mL时,酪氨酸酶抑制率最高,达25.0%。且其制成的雪花膏稳定性、光泽、颜色效果最好。抗氧化试验结果表明:桦褐孔菌总黄酮雪花膏能减少大白鼠皮肤组织匀浆中MDA的生成,增强SOD活性和羟自由基清除能力,并呈现良好的剂量-效应关系。结果说明桦褐孔菌总黄酮具有一定的抗氧化和抑制酪氨酸酶活性的能力,可以作为天然的抗衰老和美白化妆品添加剂。

Study of microwave-assisted extraction and cosmetic effect of total flavonoids from Inonotus obliquus (Fr.) Pilat

Extraction technology of total flovonoids from Inonotus obliquus(Fr.) Pilat were optimized via orthogonal designed experiment,and tyrosinase inhibition rate of the extract was measured.Varnishing cream was prepared using the extract as the active additive.Various performance tests of the varnishing cream were conducted,including determination of malonaldehyde(MDA) content,determination of SOD activity in mouse skin via pyrogallol auto-oxidation test and determination of hydroxy free radical scavenging capacity of skin tissue in vitro.Experimental results showed that the optimum conditions for extraction of flavinoids from Inonotus obliquus(Fr.) Pilat are as follows:ethanol as solvent with volume fraction of 70%;ratio of Inonotus obliquus(Fr.) Pilat material to solvent(g∶ mL),1∶ 40;microwave treatment time,120 s;microwave power,352 W.Under such conditions,the extraction yield of flavonoids from Inonotus obliquus(Fr.) Pilat achieves 4.96%.When vanishing cream base was treated by addition of 7.5 mL of the extract with mass concentration of 0.024 g·L-1 Inonotus obliquus(Fr.) Pilat flavonoids,the tyrosinase inhibition rate achieves the best condition of 25.0% and the finished product shows good appearance and stability.In addition,the antioxidation test results showed that the extract could reduce the formation of MDA,increase the enzymatic activity of SOD and hydroxyl free radical scavenging capacity in mouse skin tissue and displays good dosage-effect relationship.All results indicate that Inonotus obliquus(Fr.) Pilat flavonoids has certain antioxidation capacity and tyrosinase inhibition effect;it can be used as a cosmetic additive for skin decrepitude retarding and whitening.