Determination of Enrofloxacin in Milk Samples Using Silver Nanoparticle Enhanced Terbium-Sensitized Fluorescence Method

Terbium-sensitized fluorescence is one of the simple and sensitive methods for quantification of drugs in biological and food samples. Silver nanoparticles (AgNPs) can enhance the intensity of terbium-sensitized fluorescence and provide a more sensitive method. Enrofloxacin (ENR) is one of the common antibiotics, and the determination of its residue in animal products is recommended because of its problems for human health. Various parameters affecting the fluorescence intensity were optimized using response surface methodology. Then, the optimized parameters were applied to the validation of method for ENR quantification in milk samples. The linearity of the method was from 0.050 to 0.60 mg/L, and the limit of detection (LOD) and limit of quantification (LOQ) of the method were 0.021 to 0.069 mg/L, respectively. The accuracy, precision, and selectivity studies of the developed method give reasonable results. These data showed that the established analysis approach is a simple and sensitive technique for monitoring of ENR in milk samples.