| 大孔吸附树脂法去除车前草粗多糖中的蛋白质 |
| |
| 引用本文： | 廖春燕,杨远萍.大孔吸附树脂法去除车前草粗多糖中的蛋白质[J].食品与发酵工业,2011,37(6). |
| |
| 作者姓名： | 廖春燕 杨远萍 |
| |
| 作者单位： | 广西工学院生物与化学工程系,广西柳州,545006 |
| |
| 摘 要： | 研究AB-8大孔树脂法去除车前草粗多糖中蛋白质的适宜条件。采用动态吸附和解析实验对树脂纯化工艺进行优化。结果表明适宜工艺条件为:上样液浓度40mg/mL,上样流速0.5 mL/min,上样液pH值7.0;以蒸馏水为洗脱剂,洗脱速度2 mL/min,洗脱体积2.5BV(1BV=20 mL)。纯化后AB-8大孔吸附树脂对车前草粗多糖中的蛋白具有较高的去除效果,蛋白去除率为84.83%,多糖保留率为88.32%。
|
| 关 键 词： | 车前草 多糖 大孔吸附树脂 除蛋白 |
Study on the Removal of Protein from Plantago asiatica L.Polysaccharide by Macroporous Adsorption Resin |
| |
| Liao Chun-yan,Yang Yuan-ping.Study on the Removal of Protein from Plantago asiatica L.Polysaccharide by Macroporous Adsorption Resin[J].Food and Fermentation Industries,2011,37(6). |
| |
| Authors: | Liao Chun-yan Yang Yuan-ping |
| |
| Affiliation: | Liao Chun-yan,Yang Yuan-ping(Department of Biological and Chemical Engineering,Guangxi University of Technology,Liuzhou 545006,China) |
| |
| Abstract: | The article studied the optimal conditions of removing protein from polysaccharide of Plantago asiatica L.with AB-8 macroporous adsorption resin.The purification conditions were optimized by dynamic adsorption and desorption.The results showed that the optimum conditions were as follows:the concentration of solution was 40 mg/mL with pH of 7.0,the sample loading flow rate was 0.5 mL/min.The elution solvent was 2.5BV distilled water at flow rate of 2mL/min.AB-8 macroporous adsorption resin had the best effec... |
| |
| Keywords: | Plantago asiatica L polysaccharide macroporous adsorption resin removal of protein |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
