| 豆油和丙氨酸对利福霉素SV生物合成的影响 |
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| 引用本文： | 商纯良,王志,陈雄,王金华,黄亚男. 豆油和丙氨酸对利福霉素SV生物合成的影响[J]. 食品与发酵工业, 2011, 37(5) |
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| 作者姓名： | 商纯良 王志 陈雄 王金华 黄亚男 |
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| 作者单位： | 1. 湖北工业大学,发酵工程教育部重点实验室,湖北武汉430068
2. 河南省南街村（集团）有限公司,河南临颍,462600 |
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| 基金项目： | 中国博士后科学基金(20080430865)项目 |
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| 摘 要： | 研究了在250 mL摇瓶发酵过程中添加豆油和丙氨酸对利福霉素SV生物合成的影响。72 h分别向培养基中添加3 mL/L豆油和0.8 g/L丙氨酸,发酵液中利福霉素SV的效价分别比对照提高5.3%和6.9%。通过响应面分析法优化考察了豆油和丙氨酸对利福霉素SV合成的交互作用,确定在72 h添加0.89 g/L的丙氨酸和2.57 mL/L豆油时,摇瓶发酵单位是5 179 U/mL,比对照提高12.3%。有机酸分析结果显示,发酵培养72～120h,添加组的α-酮戊二酸和柠檬酸的利用速率分别是0.86 mg/(L.h)和0.77 mg/(L.h),分别比对照提高了115.7%和23.3%;同时,琥珀酸浓度上升到1.01 g/L,添加组比对照提高了16.1%。说明添加豆油和丙氨酸后,TCA循环由柠檬酸、α-酮戊二酸到琥珀酸转化效率提高,从而促进了琥珀酰CoA和甲基丙二酰CoA的合成,有利于利福霉素SV的合成。
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| 关 键 词： | 豆油 丙氨酸 利福霉素SV 响应面分析法 有机酸 |
Effect of Soybean Oil and Alanine Addition on Rifamycin SV Biosynthesis |
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| Shang Chun-liang,Wang Zhi,Chen Xiong,Wang Jin-hua,Huang Ya-nan ,College of Bioengineering,Hubei University of Technology,Wuhan,,China) Co.,Ltd,Linying ,China). Effect of Soybean Oil and Alanine Addition on Rifamycin SV Biosynthesis[J]. Food and Fermentation Industries, 2011, 37(5) |
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| Authors: | Shang Chun-liang Wang Zhi Chen Xiong Wang Jin-hua Huang Ya-nan College of Bioengineering Hubei University of Technology Wuhan China) Co. Ltd Linying China) |
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| Affiliation: | Shang Chun-liang1,Wang Zhi1,Chen Xiong1,Wang Jin-hua1,Huang Ya-nan2 1(Key Laboratory of Fermentation Engineering(Minister of Education),College of Bioengineering,Hubei University of Technology,Wuhan,430068,China) 2(Nanjiecun(Group) Co.,Ltd,Linying 462600,China) |
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| Abstract: | Effects of soybean oil and alanine on rifamycin SV biosynthesis in 250 mL flask were studied in this paper.When 3 mL/L soybean oil and 0.8 g/L alanine was added into the medium at 72 h,rifamycin SV production was increased by 5.3% and 6.9% over the control,respectively.Response surface methodology was used to study the interaction between soybean oil and alanine.Result showed that their optimal amount was 2.57 mL/L and 0.89 g/L,respectively.Under the optimal conditions,rifamycin SV production reached 5179 U... |
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| Keywords: | soybean oil alanine rifamycin SV response surface analysis organic acid |
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