| 星点设计-效应面法优化双水相-超声提取珍珠草多酚 |
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| 引用本文： | 高云涛,蒋进超,张宝通. 星点设计-效应面法优化双水相-超声提取珍珠草多酚[J]. 食品与发酵工业, 2011, 37(5) |
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| 作者姓名： | 高云涛 蒋进超 张宝通 |
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| 作者单位： | 云南民族大学化学与生物技术学院,云南昆明650500 国家民委-教育部共建民族药资源化学重点实验室,云南昆明650500 |
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| 基金项目： | 云南省社会发展科技计划项目(2007B148M); 云南省教育厅科学基金资助项目(06Y195C); 国家民委-教育部共建民族药资源化学重点实验室开放基金项目(MJY07010) |
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| 摘 要： | 利用超声与丙醇-硫酸铵双水相体系耦合对珍珠草多酚进行提取分离,基于单因素实验得到适宜条件:丙醇体积分数(A)60.0%、料液比(B)为1∶25.0(g∶mL)、超声时间(C)为30.0 min、(NH4)2SO4用量(D)为0.35g/mL。设计了四因素五水平的星点设计-效应面分析对提取条件进行优化,获得的最佳提取条件是:丙醇体积分数53.65%、料液比为1∶23.97(g∶mL)、超声时间为28.19 min、(NH4)2SO4用量为0.40 g/mL,珍珠草总多酚提取率E预测值为8.67%,与实验值E=8.72%(n=5)相符,优于单因素试验优化结果(8.61%,n=5)。
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| 关 键 词： | 超声 丙醇-硫酸铵双水相 提取 珍珠草 植物多酚 星点设计 效应面分析 |
Optimization of Ultrasound-assisted Aqueous Two-phase Extraction of Polyphenols from Phyllanthus urinaria L.by Central Composite Design and Response Surface Methodology |
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| Gao Yun-tao,,Jiang Jin-chao,Zhang Bao-ton. Optimization of Ultrasound-assisted Aqueous Two-phase Extraction of Polyphenols from Phyllanthus urinaria L.by Central Composite Design and Response Surface Methodology[J]. Food and Fermentation Industries, 2011, 37(5) |
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| Authors: | Gao Yun-tao Jiang Jin-chao Zhang Bao-ton |
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| Affiliation: | Gao Yun-tao1,2,Jiang Jin-chao1,Zhang Bao-ton1,2 1(School of Chemistry and Bio-technology,Yunnan University of Nationalities,Kunming 650500,China) 2(Key Lab of National Medicine Supported Jointly by State Ethnic Affairs Commission and Ministry of Education,China) |
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| Abstract: | Ultrasound-assisted propyl alcohol ammonium sulfate aqueous two-phase extraction of polyphenols from Phyllanthus urinaria L.was investigated.The best condition for the single factor experiment was: propyl-alcohol concentration 60.0%,liquid/solid ratio B 25.0,extraction time C 30min.A Response surface methodology,with a five levels,four variables central composite designs was applied based on the single factor optimization experiment.The best condition was: propyl-alcohol concentration 53.65%,liquid/solid ra... |
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| Keywords: | ultrasonic propy1-alcohol ammonium sulfate aqueous two-phase extraction Phyllanthus urinaria polyphenols L. central composite design response surface methodology |
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