| 四川香肠中产香葡萄球菌的分离与鉴定 |
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| 引用本文： | 杨勇,张雪梅,程艳,刘书亮,李诚,秦文,蒲彪.四川香肠中产香葡萄球菌的分离与鉴定[J].食品与发酵工业,2011,37(6). |
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| 作者姓名： | 杨勇 张雪梅 程艳 刘书亮 李诚 秦文 蒲彪 |
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| 作者单位： | 四川农业大学食品学院,四川雅安,625014 |
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| 基金项目： | 四川省教育厅项目(07ZS004) |
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| 摘 要： | 从四川自然发酵香肠中共分离得到242株微球菌和葡萄球菌,采用快速半定量平板方法从中筛选出了硝酸盐还原酶活性较大的株菌80株,并对这些菌株进行发酵基本实验和产香基本要求实验,筛选得到了2株过氧化氢酶阳性葡萄球菌S15和S25。通过GC-MS分析发现,在实验条件下只有S25具有代谢亮氨酸生成3-甲基丁醛的能力。生化鉴定以及菌株的16S rDNA测序结果表明,S25为腐生葡萄球菌。
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| 关 键 词： | 四川香肠 葡萄球菌 产香能力 分离 鉴定 |
Isolation and Identification of Aroma Producing Staphylococci from Sichuan Sausage |
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| Yang Yong,Zhang Xue-mei,Cheng Yan,Liu Shu-liang,Li Cheng,Qin Wen,Pu Biao.Isolation and Identification of Aroma Producing Staphylococci from Sichuan Sausage[J].Food and Fermentation Industries,2011,37(6). |
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| Authors: | Yang Yong Zhang Xue-mei Cheng Yan Liu Shu-liang Li Cheng Qin Wen Pu Biao |
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| Affiliation: | Yang Yong,Zhang Xue-mei,Cheng Yan,Liu Shu-liang,Li Cheng,Qin Wen,Pu Biao(College of Food Science,Sichuan Agricultural University,Ya'an 625014,China) |
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| Abstract: | A total of 242 strains of Micrococcaceae and Staphylococcus were isolated from Sichuan natural fermented sausages.80 strains which have high nitrate reduction properties were selected by a fast semi-quantitative plate assay method.Different experiments were carried out according to the common selection criteria of starter culture and aroma production.Two strains of Staphylococcus named S15 and S25 were obtained with the ability to produce catalase.GC-MS analysis showed that only S25 produced 3-methyl-butana... |
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| Keywords: | Sichuan sausage Staphylococcus capability of aroma production isolation identification |
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