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松花粉提取物抗氧化活性及其酚类化合物的研究
引用本文:程妮,高慧,王毕妮,邓建军,刘媛媛,曹炜. 松花粉提取物抗氧化活性及其酚类化合物的研究[J]. 食品与发酵工业, 2011, 37(5)
作者姓名:程妮  高慧  王毕妮  邓建军  刘媛媛  曹炜
作者单位:西北大学食品科学与工程系,陕西西安,710069
基金项目:西安市科技计划项目(NC1008(18))
摘    要:为研究松花粉抗氧化活性,采用HPLC-ECD方法测定了松花粉的水提取物和乙醇(乙醇体积分数为80%)提取物中的酚类化合物的含量。同时评价了其对羟基自由基介导的2-脱氧核糖裂解的保护作用。结果表明:2种提取物中均含有原儿茶酸、咖啡酸、p-香豆酸、阿魏酸和高良姜素,乙醇提取物中含有芦丁、槲皮素和山奈酚。松花粉乙醇提取物中的总酚含量显著高于水提物(P<0.05),但水提取物的还原能力和络合能力显著高于乙醇提取物(P<0.05)。2种提取物对非定位羟基自由基介导的2-脱氧核糖裂解的保护作用均显著高于定位羟基自由基(P<0.05),表明松花粉提取物对羟基自由基介导的2-脱氧核糖裂解的保护作用主要通过清除羟基自由基来实现的,这可能与松花粉提取物中含有大量的酚类化合物有关。

关 键 词:松花粉  提取物  抗氧化  酚类化合物

Antioxidant Activities and Phenolic Compounds of the Pine Pollen Extracts
Cheng Ni,Gao Hui,Wang Bini,Deng Jian-jun,Liu Yuan-yuan,Cao Wei. Antioxidant Activities and Phenolic Compounds of the Pine Pollen Extracts[J]. Food and Fermentation Industries, 2011, 37(5)
Authors:Cheng Ni  Gao Hui  Wang Bini  Deng Jian-jun  Liu Yuan-yuan  Cao Wei
Affiliation:Cheng Ni,Gao Hui,Wang Bini,Deng Jian-jun,Liu Yuan-yuan,Cao Wei(Department of Food Science and Engineering,Northwest University,Xi'an 710069,China)
Abstract:Antioxidant activities of the pine pollen extracts was studied.Phenolics were extracted from the pine pollen using water and 80% ethanol as solvents.The extracts are analyzed by HPLC-ECD.And the scavenging effects of extracts on hydroxyl radical-mediated 2-deoxyribose degradation were studied.The results revealed that protocatechuic acid,caffeic acid,p-coumaric acid,ferulic acid and galangin could be identified and quantified in both extracts.Rutin,quercetin and kaempferol were identified and quantified in ...
Keywords:pine pollen  extracts  antioxidant activities  phenolic compounds  
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