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营养条件对绿僵菌生长特性和抑菌活性的影响
引用本文:齐永霞,陈方新,李增智. 营养条件对绿僵菌生长特性和抑菌活性的影响[J]. 食品与发酵工业, 2011, 37(7)
作者姓名:齐永霞  陈方新  李增智
作者单位:1. 安徽农业大学植物保护学院,安徽合肥,230036
2. 安徽农业大学微生物防治重点实验室,安徽合肥,230036
基金项目:国家自然科学基金(30972368)
摘    要:研究了不同培养基、碳、氮营养对金龟子绿僵菌菌落直径、菌丝生物量、分生孢子产生量及其次生代谢产物对植物病原真菌的抑菌活性。发现金龟子绿僵菌的菌丝线性生长以PSA、麦芽糖和酵母最好,以Czapek、甘露醇和脲最差;菌丝生物量以PSA、白砂糖和蛋白胨最大,以Czapek、乳糖和脲最小;分生孢子产生量以PSA、麦芽糖和KNO3最多,以Czapek、甘油和硫酸铵最少。代谢液的抑菌活性实验结果表明,在不同培养基培养的代谢液中,Czapek的抑菌效果最好,SDAY的抑菌效果最差。在不同碳营养培养的代谢液中,白砂糖的抑菌效果最好,乳糖的抑菌效果最差。在不同氮营养培养的代谢液中,蛋白胨的抑菌效果最好,脲的抑菌效果最差。

关 键 词:碳营养  氮营养  金龟子绿僵菌  生物学特性  抑菌活性

Effects of Different Nutrition on the Biological Characteristics and Antifungal Activity of Metarhizium anisopliae
Qi Yong-xia,Chen Fang-xin,Li Zeng-zhi. Effects of Different Nutrition on the Biological Characteristics and Antifungal Activity of Metarhizium anisopliae[J]. Food and Fermentation Industries, 2011, 37(7)
Authors:Qi Yong-xia  Chen Fang-xin  Li Zeng-zhi
Affiliation:Qi Yong-xia,Chen Fang-xin,Li Zeng-zhi 1(College of Plant Protection,Anhui Agricultural University,Hefei 230036,Anhui,China) 2(Anhui Provincial Key Laboratory for Microbial Pest Control,China)
Abstract:The biological characteristics of Metarhizium anisopliae were compared with different culture media,carbon sources and nitrogen sources by measuring the mycelium growth,dry mycelium biomass and conidia production.The results showed that the optimum culture media,carbon source and nitrogen source for the mycelia growth of M.anisopliae was PSA,maltose and yeast,respectively.The PSA,white granulated sugar and peptone were the suitable media,carbon sources and nitrogen sources for the biomass accumulation,which...
Keywords:carbon nutrition  nitrogen nutrition  Metarhizium anisopliae  biological characteristics  anti-fungal activity  
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