凝胶渗透色谱净化系统与气相色谱-串联质谱法检测5种食品中14种有机磷和7种拟除虫菊酯类农药残留

建立GPC与GC-MS/MS测定5种食品中14种有机磷和7种拟除虫菊酯类农药残留的方法,初步分析GPC净化技术及MS/MS分析技术在食品农药多残留分析的优势。方法 用GPC作为样品前处理方法,处理韭菜、大白菜、辣椒、猪肉、鱼肉五种食品,用 GC-MS/MS检测并进行定性、定量分析。结果 GPC与GC-MS-MS检测食品中14种有机磷和7种拟除虫菊酯类农药方法的线性相关系数r＞0.995,定量限为0.002～0.034 mg/kg,对韭菜、猪肉样品按0.05、0.10、0.20 mg/kg三组水平加标测试,方法精密度RSD%为2.9～10.2%,回收率为78.6～108.3%。结论 本方法检测样品范围广、灵敏度高、定性可靠、定量准确,适合多种类食品中农药多残留分析。

Determination of 14 organophosphorus pesticides and 7 pyrethroids pesticides in five kinds of food by GPC and GC-MS/MS

To establish a method for determining 14 organophosphorus pesticides(OPs) and 7 pyrethroids pesticides (PPs) in 5 kinds of food by GPC and GC-MS/MS, and find the advantages of GPC and GC-MS/MS in analyzing pesticide residues in food.MethodsFive kinds of food including leeks, Chinese cabbage, pepper, pork and fish were pretreated with GPC, and the samples were analyzed qualitatively and quantitatively by GC-MS/MS. ResultsThe linear correlation coefficients of 14 OPs and 7 PPs were above 0.995. The limit of quantification was in the range of 0.002-0.034 mg/kg. The relative standard deviation (RSD) was 2.9-10.2%, and the rates of recovery were 78.6-108.3% for leek and pork samples spiked with 0.05,0.10 and 0.20 mg/kg of 14 OPs and 7 PPs. ConclusionThe method was widely used, highly sensitive, reliable, accurate, and suitable for analysis of pesticide residues in many kinds of food.