一起食物中毒病因的实验室分析

查明引起食物中毒的致病菌,分析菌株间的亲缘关系,为明确食物中毒诊断提供依据。方法 采集一起食物中毒蛋糕和病人样品,用实时荧光PCR快速筛检,按GB 4789.4—2010《食品微生物学检验 沙门菌检验法》分离、鉴定致病菌,脉冲电场凝胶电泳(PFGE)对病原菌作同源性分析。结果 从16份蛋糕样品中检出8株肠炎沙门菌,32份患者粪便样品中检出17株肠炎沙门菌,PCR核酸阳性与GB 4789.4—2010法分离到菌株完全一致；PFGE条带聚类分析显示患者与蛋糕中检出的肠炎沙门菌属同一基因型,具有高度同源性。结论 本起食物中毒由肠炎沙门菌污染蛋糕所致；PCR法与GB 4789.4—2010法联合检测,有助于快速锁定食物中毒致病菌,从基因水平上证明食物病原的相关性。

The laboratory analysis of a food poisoning pathogeny

Identify pathogens which causing food poisoning, analysis of genetic relationships among strains and provide the basis for the diagnosis of food poisoning clear.MethodsUsing quantitative PCR rapid screening, GB 4789.4-2010 method to separate and identify the pathogens. ResultsFrom the 16 cake samples detected 8 Salmonella enteritidis, 32 stool samples of patients detected 17 Salmonella enteritidis, PCR nucleic acid positive and the GB 4789.4-2010 method were isolated with the same strains. PFGE banding showed that patients had the same pattern of Salmonella enteritidis with which were detected in cake, and they had high homology.ConclusionThis food poisoning from Salmonella enteritidis contamination by the cake. PCR method with the GB 4789.4-2010 method helps to quickly lock joint detection of food poisoning bacteria. PFGE detected Salmonella enteritidis of the cake and patients were sourced from the same clone. It demonstrate the relevance of food pathogens from genes level.