亲代暴露致敏原对子代BN大鼠致敏模型的影响

研究BN大鼠亲代暴露致敏原后子代对致敏原免疫反应变化。方法 亲代分成暴露与非暴露致敏原,两组所产大鼠均随机分为卵清蛋白(OVA)组、空白对照组,分别每日经口给予1 mg/ml OVA溶液、灭菌蒸馏水1 ml,共6周；第2周、第4周、第6周取血分离血清,测定特异性抗体IgG和IgE；第3天和第5天取血分离血浆,测定组胺；最后测定两组胃肠道渗透性。结果 亲代非暴露致敏原的大鼠可激发较强的过敏反应,特异性IgG、特异性IgE、组胺水平均升高；亲代暴露致敏原的大鼠激发后免疫反应较弱。结论 BN大鼠亲代接触致敏原后,其子代致敏免疫反应减弱。

Effect of parental rat exposure to allergen on the sensitization model of filial generation in BN rat

To study the immune response of offspring rats after parental rats exposure to allergen. MethodsParental BN rats were divided exposure and non-exposure to allergen groups. The offspring rats were randomly divided into OVA group, control group respectively, OVA and control were daily administrated at the dose of 1 mg/ml OVA or at the dose of sterile water by gavage for 6 weeks. Blood samples were taken on the 2^nd, 4^th, and 6^thweeks from the orbital plexus, and centrifuged to obtain sera for analyzing specific IgG and IgE.On the 3^rd, 5^thweeks, blood samples were taken to obtain plasma for analyzing histamine. In addition, the changes of gut permeability were determined.ResultsAllergenic responsecould be induced by provoke allergen OVA in BN rats when parental rats were non-exposure to allergen OVA, include specific IgG,IgE increasing and histamine increasing. However, little allergenic response be provoked by OVA in BN rat when parental rats were exposure to allergen OVA.ConclusionThe immuneresponse of offspring BN rats were weaken after parental rats exposure to allergen.