浙贝母精油化学成分GC/MS分析和抑菌活性检测 |
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引用本文: | 曹跃芬,竺锡武,谭琳.浙贝母精油化学成分GC/MS分析和抑菌活性检测[J].浙江丝绸工学院学报,2012(1):129-132. |
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作者姓名: | 曹跃芬 竺锡武 谭琳 |
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作者单位: | [1]浙江理工大学生物工程研究所,杭州310018 [2]湖南农业大学,长沙410128 |
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基金项目: | 基金项目:浙江省重中之重学科开放基金项目(SWYX0911);国家自然科学基金项目(31071729) |
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摘 要: | 采用水蒸汽蒸馏法提取浙贝母精油,GC/MS分析法结合保留指数分析法鉴定精油化学成分,琼脂扩散法检测精油的抑菌活性。首次鉴定出浙贝母精油的15种化学成分,占精油总离子流色谱峰面积的97.48%,主要成分为正十六酸(53.46%),(E,E)-9,12-十八烷二烯酸甲酯(26.96%)和油酸(9.34%)。浙贝母精油对金黄色葡萄球菌和大肠杆菌均无抑制效果,对白色念珠茵有中等抑制作用。
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关 键 词: | 浙贝母 精油 GC/MS Kovats保留指数 抑菌活性 |
Analysis of Chemical Composition by GO/MS and Antimicrobial Activities of Essential Oil from Fritillaria thunbergii Miq. |
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Authors: | CAO Yue-fen ZHUXi-wu TAN Lin |
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Affiliation: | 1. Institute of Bioengineering, Zhejiang Sci-Tech University, Hangzhou 310018, China; 2. Hunan Agricultural University, Changsha 410128, China) |
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Abstract: | The essential oil from dried bulbs of Fritillaria thunbergii Miq. obtained by hydrodistil lation is analyzed by GC-MS together with Kovats retention index. The results show that major constituents of this oil are n-Hexadecanoic acid (53. 46%), (E, E)-9, 12-Octadecadienoic acid (26.96%) and Oleic Acid (9.34%) and 15 chemical components are identified which represents 97. 5% of the total peak. The result tested by agar diffusion method shows that the oil has a moderate antifungal activity against Canidia albicans and no antimicrobial activity to Escherichia coli and Staphylococcus aureus. |
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Keywords: | Fritillaria thunbergii Miq essential oil GC/MS Kovats retention index antimicrobial activity |
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