| 浙贝母精油化学成分GC／MS分析和抑菌活性检测 |
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| 引用本文： | 曹跃芬,竺锡武,谭琳. 浙贝母精油化学成分GC／MS分析和抑菌活性检测[J]. 浙江丝绸工学院学报, 2012, 0(1): 129-132 |
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| 作者姓名： | 曹跃芬 竺锡武 谭琳 |
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| 作者单位： | [1]浙江理工大学生物工程研究所,杭州310018 [2]湖南农业大学,长沙410128 |
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| 基金项目： | 基金项目：浙江省重中之重学科开放基金项目（SWYX0911）;国家自然科学基金项目（31071729） |
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| 摘 要： | 采用水蒸汽蒸馏法提取浙贝母精油，GC／MS分析法结合保留指数分析法鉴定精油化学成分，琼脂扩散法检测精油的抑菌活性。首次鉴定出浙贝母精油的15种化学成分，占精油总离子流色谱峰面积的97．48％，主要成分为正十六酸（53．46％），（E，E）－9，12－十八烷二烯酸甲酯（26．96％）和油酸（9．34％）。浙贝母精油对金黄色葡萄球菌和大肠杆菌均无抑制效果，对白色念珠茵有中等抑制作用。
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| 关 键 词： | 浙贝母 精油 GC／MS Kovats保留指数 抑菌活性 |
Analysis of Chemical Composition by GO/MS and Antimicrobial Activities of Essential Oil from Fritillaria thunbergii Miq. |
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| CAO Yue-fen ZHUXi-wu,TAN Lin. Analysis of Chemical Composition by GO/MS and Antimicrobial Activities of Essential Oil from Fritillaria thunbergii Miq.[J]. , 2012, 0(1): 129-132 |
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| Authors: | CAO Yue-fen ZHUXi-wu TAN Lin |
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| Affiliation: | 1. Institute of Bioengineering, Zhejiang Sci-Tech University, Hangzhou 310018, China; 2. Hunan Agricultural University, Changsha 410128, China) |
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| Abstract: | The essential oil from dried bulbs of Fritillaria thunbergii Miq. obtained by hydrodistil lation is analyzed by GC-MS together with Kovats retention index. The results show that major constituents of this oil are n-Hexadecanoic acid （53. 46%）, （E, E）-9, 12-Octadecadienoic acid （26.96%） and Oleic Acid （9.34%） and 15 chemical components are identified which represents 97. 5% of the total peak. The result tested by agar diffusion method shows that the oil has a moderate antifungal activity against Canidia albicans and no antimicrobial activity to Escherichia coli and Staphylococcus aureus. |
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| Keywords: | Fritillaria thunbergii Miq. essential oil GC/MS Kovats retention index antimicrobial activity |
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