Lipase-catalyzed acidolysis of menhaden oil with pinolenic acid |
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Authors: | In-Hwan Kim Charles G Hill Jr |
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Affiliation: | (1) Department of Food and Nutrition, College of Health Sciences, Korea University, 136-703 Seoul, Korea;(2) Dept. of Chemical and Biological Engineering, University of Wisconsin-Madison, 1415 Engineering Dr., 53706 Madison, WI |
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Abstract: | Lipase-catalyzed acidolysis of menhaden oil with a pinolenic acid (PLA) concentrate, prepared from pine nut oil, was studied
in a solvent-free system. The PLA concentrate was prepared by urea complexation of the FA obtained by saponification of pine
nut oil. Eight commercial lipases from different sources were screened for their ability to catalyze the acidolysis reaction.
Two different types of structured lipids (SL) were synthesized. The first type, which has PLA residues as a primary FA residue
at the sn-1,3 positions of the TAG, was synthesized using a 1,3-regiospecific lipase, namely, Lipozyme RM IM from Rhizomucor miehei. The second type of SL, which has PLA residues as a primary FA residue at both the sn-1,3 and sn-2 positions of the TAG, was synthesized using a nonspecific lipase, namely, Novozym 435 from Candida antarctica. The effects of variations in enzyme loading, temperature, and reaction time on PLA incorporation into the oil were monitored
by GC analyses. The optimal temperature and enzyme loading for synthesis of the two types of SL were 50°C and 10% of the total
weight of substrates for both enzymes. The optimal reaction time for the synthesis with Lipozyme RM IM was 16h, whereas the
optimal reaction time for the synthesis mediated by Novozym 435 was 36 h. Pancreatic lipase-catalyzed sn-2 positional analyses were also carried out on the TAG samples. |
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Keywords: | Acidolysis lipase menhaden oil pinolenic acid (PLA) pine nut oil urea complexation |
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