罗布麻中黄酮的超临界CO2萃取及其抗菌性

为解决传统脱胶工艺罗布麻韧皮纤维中黄酮化合物的浪费问题，对罗布麻韧皮纤维采取超临界脱胶预处理的同时，利用超临界CO2 提取其黄酮有效成分，研究分析影响罗布麻中黄酮萃取的4个主要因素，然后对萃取液进行了高效液相色谱分析和抗菌实验。结果表明：助溶剂主要影响超临界流体的极性，随着助溶剂含量的增加，超临界流体极性的增加提高了黄酮萃取率，但助溶剂含量过高时，过多水分易造成萃取管路堵塞，使黄酮的萃取效率降低；在45 ℃、20 MPa时黄酮萃取率较高，升高或降低压力与温度均会影响黄酮萃取效果；随着超临界CO2流量的增加，黄酮萃取率逐渐增加，但对萃取设备要求更高；罗布麻中黄酮化合物具有良好的抗菌效果，是罗布麻纤维具有抗菌性能的原因之一。

Supercritical carbon dioxide extraction and bacterial resistance of flavones from apocynum venetum bast fiber

In order to solve the problem of the waste of flavones compounds in apocynum venetum bast fiber in conventional chemical degumming process, flavones from apocynum venetum bast fiber before degumming were extracted with supercritical carbon dioxide. The influence of cosolvent content, temperature, pressure and time on the extraction of flavones was investigated. Then the extracted solution was subjected to high performance liquid chromatographic analysis and antibacterial experiments. The results show that cosolvent mainly influences the polarity of supercritical fluid, and with the increaseof solvent content, the polarity of the supercritical fluid increases the extraction rate. If the cosolvent content is too high, excessive water may cause the extraction pipeline blockate, which will decrease the extraction efficiency. Under the conditions of45 °C and 20 MPa, the extraction rate of flavonoids is higher.Both of theincrease and decrease of pressure and temperature will influence the extraction dfficiency of flavonoids. With the increase of the flow rate of supercritical carbon dioxide, the extraction rate of flavonoids increased gradually, but the requirement for equipment is high. Moreover, flavones compounds in apocynum venetum bast fiber have good antibacterial effect, which endues apocynum venetum bast fiber with good antibacterial performance.