Development of a compact high-density microbial hydrogen reactor for portable bio-fuel cell system |
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Authors: | Mitsuyoshi Ishikawa Shohei Yamamura Yuzuru Takamura Koji Sode Eiichi Tamiya Masamitsu Tomiyama |
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Affiliation: | 1. Japan Advanced Institute of Science and Technology, School of Materials Science, 1-1, Asahidai, Nomi City, Ishikawa 923-1292, Japan;2. Department of Life Science and Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan;3. National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan |
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Abstract: | The development of a compact high-density microbial reactor for hydrogen production is described with possible implications to use as a portable bio-fuel cell system. To construct the compact bioreactor, mainly, the cell density and immobilization methods were optimized in this paper. The encapsulation of hydrogen producing bacterium, Escherichia coli strain MC13-4, in alginate gel beads provided approximately three-fold increase in hydrogen production in comparison with the free cell suspension. The immobilized cells (cell density; O.D. 100) and 500 mM glucose solution were packed into a 20 mL glass bottle that was connected to the fuel cell. This system has generated electricity of over 20 mW for 20 min. |
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Keywords: | Biohydrogen Bioreactor Cell immobilization Bio-fuel cell |
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