| 大肠杆菌中同时表达的可溶性和不溶性人α-干扰素-2b的初步分离研究 |
| |
| 引用本文： | 徐志南,岑沛霖.大肠杆菌中同时表达的可溶性和不溶性人α-干扰素-2b的初步分离研究[J].中国化学工程学报,2002,10(6):686-689. |
| |
| 作者姓名： | 徐志南 岑沛霖 |
| |
| 作者单位： | Bioengineering Research Institute，Department of Chemical Engineering and Bioengineering，Zhejiang University，Bioengineering Research Institute，Department of Chemical Engineering and Bioengineering，Zhejiang University Hangzhou 310027，China，Hangzhou 310027，China |
| |
| 摘 要： | 1 INTRODUCTION Interferons are proteins naturally occurring in the hu-man body with antiviral, antiproliferative and immu-noregulatory activity1]. The commercial production of interferons as important clinic medicines was carried out by using recombinant E. coli system2]. Despite the ease of cloning and genetic manipulation in E. coli, heterogeneous protein was almost expressed as inclusion body form since the outer membrane appears to be a very effective barrier to the release of pro…
|
| 关 键 词： | 大肠杆菌 同时表达 α-干扰素-2b 净化 蛋白质 分离 可溶性 不溶性 |
| 修稿时间： | |
Primary Purification of Co-expressed Soluble and Insoluble Alpha-interferon 2b from Recombinant E.coli |
| |
| XU Zhinan,CEN Peilin.Primary Purification of Co-expressed Soluble and Insoluble Alpha-interferon 2b from Recombinant E.coli[J].Chinese Journal of Chemical Engineering,2002,10(6):686-689. |
| |
| Authors: | XU Zhinan CEN Peilin |
| |
| Affiliation: | Bioengineering Research Institute, Department of Chemical Engineering and Bioengineering, Zhejiang University, Hangzhou 310027, China |
| |
| Abstract: | Alpha-interferon 2b (IFN 2b) was produced both in soluble and insoluble forms from |
| |
| Keywords: | alpha-interferon 2b soluble expression inclusion body refolding purification * |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国化学工程学报》浏览原始摘要信息 |
|
点击此处可从《中国化学工程学报》下载全文 |
