| 用FA和ECM方法测定吸附等温线的比较 |
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| 引用本文： | Ju-Weon LEE,Kyung Ho ROW. 用FA和ECM方法测定吸附等温线的比较[J]. 中国化学工程学报, 2002, 10(6): 720-722 |
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| 作者姓名： | Ju-Weon LEE Kyung Ho ROW |
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| 作者单位： | CenterforAdvancedBioseparationTechnology,DepartmentofChemicalEgineering,InhaUniversity,Incheon402-751,Korea |
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| 摘 要： | Adsorption isotherm is the most fundamental information related to chromatography.To calculate the parameters of Langmuir adsorption isotherm of thymidine,fronal analysis(FA)and lution-curve method(ECM)were adopted in reversed-phase high performance liguid chromatography(RP-HPLC).In FA,tha concentration of stationary phase was measured from the elution curves and the isotherm was determined by regression analysis,while the parameters by ECM were obtained by parameter optimization.The adsorption isotherms of thymidine from the two methods were very similar.The superiority of ECM over FA was that the consumption of sample was less and only one or two in jections of sample were required.
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| 关 键 词： | 前沿分析法 测定 吸附等温线 比较研究 洗脱曲线法 高效液相色谱 |
| 修稿时间： | |
Comparison of Frontal Analysis and Elution-Curve Method for Determining Adsorption Isotherms |
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| Ju-Weon LEE,Kyung Ho ROW. Comparison of Frontal Analysis and Elution-Curve Method for Determining Adsorption Isotherms[J]. Chinese Journal of Chemical Engineering, 2002, 10(6): 720-722 |
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| Authors: | Ju-Weon LEE Kyung Ho ROW |
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| Affiliation: | Center for Advanced Bioseparation Technology, Department of Chemical Engineering, Inha University, Incheon 402-751, Korea |
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| Abstract: | Adsorption isotherm is the most fundamental information related to chromatography. To calculate the parameters of Langmuir ad-sorption isotherm of thymidine, frontal analysis (FA) and elution-curve method (ECM) were adopted in reversed-phase high performance liguid chromatography (RP-HPLC). In FA, the concentration of stationary phase was measured from the elution curves and the isotherm was deter-mined by regression analysis, while the parameters by ECM were obtained by parameter optimization. The adsorption isotherms of thymidine from the two methods were very similar. The superiority of ECM over FA was that the consumption of sample was less and only one or two in-jections of sample were required. |
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| Keywords: | frontal analysis elution-curve method adsorption isotherm reversed-phase high performance liguid chromatography * |
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