Isolation and characterization of a major allergen in kiwi fruit |
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| Authors: | Manfred M?ller Marlies Kayma H. Steinhart Angelika Paschke |
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| Affiliation: | (1) University of Hamburg, Institute of Biochemistry and Food Chemistry, Department of Food Chemistry, Grindelallee 117, D-20146 Hamburg, Germany, DE;(2) Hospital Borkum Riff of the BfA, Department of Dermatology and Allergy, Hindenburgstrasse 126, D-26757 Borkum, Germany, DE |
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| Abstract: | The isolation of an important allergen in kiwi fruit (Actinidia chinensis) by ion-exchange chromatography (IEC) and micropreparative SDS-PAGE followed by electroelution is reported. The purity of the allergen was analysed by SDS-PAGE and immunoblotting with sera from patients who have an allergy to kiwi. The allergen was shown to have a molecular weight of 43 kDa by SDS-PAGE/immunoblotting and an isoelectric point of approximately 6.9 as estimated by IEC. In accordance with World Health Organization nomenclature, this allergen is called Act c 2. By immunoblot inhibition it was shown that epitopes from different allergens in kiwi fruit are also located on Act c 2. N-terminal amino acid sequencing of 17 amino acid residues did not reveal homology with the major allergens in birch pollen (Bet v 1), apple (Mal d 1) or with other proteins of allergenic plant foods. In addition, the isoelectric point of a 67-kDa allergen in kiwi fruit was estimated to be 7.4 by IEC, but micropreparative isolation of this allergen failed because of its very low content in the fruit. Received: 14 April 1997 |
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| Keywords: | Food allergens Immunoblotting Ion-exchange chromatography Kiwi fruit N-terminal microsequencing |
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