桦褐孔菌深层发酵培养条件的优化研究

以多糖和生物量的得率为指标,研究了不同碳源、氮源、无机盐、温度、初始pH值、接种量等不同因素对桦褐孔菌深层发酵的影响,运用了单因素试验的方法探索了其最佳的液体培养条件。确定了促进桦褐孔菌菌丝体发酵生产多糖的培养基组成为1.8%蔗糖,0.2%麸皮,0.05%MgSO.47H2O和0.1%KH2PO4。适宜发酵多糖的条件为:发酵温度25℃,培养基初始pH值为8.5,接种量为10%。

Optimization of submerged fermentation conditions of Inonotus obliquus

Using biomass and polysaccharide yield as indicators,effects of different factors,such as carbon sources,nitrogen sources,inorganic salts,temperature,initial pH value and inoculation amount,on submerged fermentation of Inonotus obliquus were studied.Single-factor experimental design was used for optimum fermentation conditions.The optimum fermentation medium for polysaccharide production was obtained as follows： sucrose 1.8%,wheat bran 0.2%,KH2PO4 0.1% and MgSO4·7H2O 0.05% and optimum fermentation conditions were initial pH value 8.5,inoculation amount 10%（v/v） and fermentation temperature 25℃.