Effect of low-molecular-weight silk fibroin peptides on cell growth property北大核心CSCD

The main purpose of this paper is to analyze the effects of low-molecular-weight silk fibroin peptides (LMW-SF) on cell growth characteristics. Firstly, LMW-SF was achieved from silk fibroin enzymolysis by neutral protease. Secondly, LMW-SF was sterilized by filtration and high-pressure steam, respectively. Finally, the effects of LMW-SF on the growth of human normal liver cell (L02) and human liver cancer cell (HepG2) were tested under the volume concentration of 0. 1 ∼5.0 mg/mL, and the relative growth rate (RGR) was calculated. The results show that LMW-SF have slight effect on cell growth at low concentration (0. 1 mg/mL and 0. 5 mg/mL); when the volume concentration is high (2. 5 mg/mL and 5. 0 mg/mL), the lowest value of RGR is 8. 3%, indicating a strong inhibition effect on cell growth. RGR is up to 227. 4% after high-pressure steam sterilization, showing a strong promotion effect on cell growth. It is obvious that LMW-SF have a strong effect on promoting cell growth after highpressure steam sterilization, and high-pressure steam sterilization could be a necessary process when protein is applied in tissue engineering scaffolds. © 2017 China Silk Association. All rights reserved.