Reinvestigation of positional distribution of fatty acids in docosahexaenoic acid-rich fish oil triacyl-sn-glycerols |
| |
Authors: | Yasuhiro Ando Motoi Satake Yoichi Takahashi |
| |
Affiliation: | (1) Department of Marine Bioresources Chemistry, Faculty of Fisheries, Hokkaido University, 041-8611 Hakodate, Japan |
| |
Abstract: | Positional distribution of fatty acids in triacyl-sn-glycerols of docosahexaenoic acid (DHA)-rich tuna orbital and bonito head oils has been reanalyzed by a method based on chromatographic
separation of isomeric and enantiomeric monoacyl-sn-glycerol (MAG) derivatives. When boric acid thinlayer chromatography (TLC) was used for separation of 1(3)- and 2-MAG analytical
intermediates, the stereospecific analysis showed the preferential association of DHA to the sn-2 position followed by the sn-3 position. This distribution pattern differed from that obtained by silicic acid LTC of their bis-3,5-dinitrophenylurethane
(DNPU) derivatives. Reversed-phase high-performance liquid chromatography elution profiles of 1(3)- and 2-MAG intermediates
revealed that 1(3)- and 2-MAG made up of both short- and long-chain lengths cannot be clearly resolved by TLC after preparation
of the DNPU derivatives. The 1(3)- and 2-MAG must be resolved by boric acid TLC prior to derivatization. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |