| 用分子生物学手段控制啤酒中乙醛含量的研究 |
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| 引用本文： | 赵丽娟,王德良,程玉来,周建中,葛邦国. 用分子生物学手段控制啤酒中乙醛含量的研究[J]. 酿酒科技, 2006, 0(1): 45-47 |
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| 作者姓名： | 赵丽娟 王德良 程玉来 周建中 葛邦国 |
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| 作者单位： | 沈阳农业大学食品学院,辽宁,沈阳,110161;中国农业大学食品学院,北京,100009;中国食品发酵工业研究院酿酒工程部,北京,100027;新疆农业大学食品学院,新疆,乌鲁木齐,830052 |
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| 摘 要： | 以F718，R719为引物，以质粒pFA6a—kanMX4为模板进行PCR扩增，采用基因转化法获得1株乙醇脱氢酶Ⅱ基因突变型工业酿酒酵母。驯养后的突变株啤酒生产小试表明，突变株乙醛含量为5．386mg／L，比原菌株乙醛含量7．932mg／L有所降低；发酵液发酵结束时，双乙酰含量为0．058mg／L，比原菌株双乙酰含量0．034mg／L有所升高；突变株发酵度为63％，比原菌株66％略有降低。
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| 关 键 词： | 分子生物学 啤酒 乙醛含量 啤酒酵母 |
| 文章编号： | 1001-9286(2006)01-0045-03 |
| 收稿时间： | 2005-08-19 |
| 修稿时间： | 2005-08-19 |
Study on the Control of Aldehyde Content in Beer by Molecular Biological Measures |
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| ZHAO Li-juan,WANG De-liang,CHENG Yu-lai,ZHOU Jian-zhong,GE Bang-guo. Study on the Control of Aldehyde Content in Beer by Molecular Biological Measures[J]. Liquor-making Science & Technology, 2006, 0(1): 45-47 |
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| Authors: | ZHAO Li-juan WANG De-liang CHENG Yu-lai ZHOU Jian-zhong GE Bang-guo |
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| Affiliation: | 1.Food Science College of Shenyang Agricultural University, Shenyang 110161 ; 2. Food Science College of China Agricultural University, Beijing 100009; 3. Wine-making Engineering Department of China Food Fermentation Industry Research Institute, Beijing 100027; 4. Food Science College of Xinjiang Agricultural University, Urumchi 830052, China |
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| Abstract: | With F718 and F719 used as primer and plasmid pFa6a-kanMX4 used as template for PCR amplification,one S.cerevisiae strain of alcohol dehydrogenase II gene mutation type was obtained through genetic transformation methods.The application of domesticated mutant strain in beer production suggested that aldehyde content in beer produced by mutant strain was 5.386 mg/L(aldehyde content in beer by original strain was 7.932 mg/L),the content of diacetyl was 0.058 mg/L at the end of liquid fermentation(diacetyl content in beer by original strain was 0.034 mg/L),and the fermenting degree of mutant reached 63 %(the fermenting degree of original strain was 66 %).(Tran.by YUE Yang) |
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| Keywords: | molecular biology beer aldehyde content beer yeast |
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