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食品中生物毒素的ELISA分析方法研究进展
引用本文:林壮森,张焜,赵肃清,郑劼,黄宝华. 食品中生物毒素的ELISA分析方法研究进展[J]. 食品科学, 2009, 30(3): 281-283. DOI: 10.7506/spkx1002-6630-200903063
作者姓名:林壮森  张焜  赵肃清  郑劼  黄宝华
作者单位:广东工业大学轻工化工学院
摘    要:由生物毒素引起的食物中毒引起各国政府以及广大消费者的普遍关注。酶联免疫吸附法(enzyme-linked immunosorbent assay,ELISA)是一种把抗原和抗体的特异性免疫反应和酶的高效催化作用有机结合起来的检测技术。本文重点从方法的线性范围、检出限、回收率等方面概述近年来ELISA 法在食品中生物毒素(微生物毒素、动物毒素、植物毒素)检测的应用,旨在促进该技术在食品安全检测中的应用和推广。

关 键 词:酶联免疫吸附分析  生物毒素  食品  检测  
收稿时间:2008-01-17

Research Progress on ELISA Method for Analysis of Biotoxins in Foodstuffs
LIN Zhuang-sen,ZHANG Kun,ZHAO Su-qing,ZHENG Jie,HUANG Bao-hua. Research Progress on ELISA Method for Analysis of Biotoxins in Foodstuffs[J]. Food Science, 2009, 30(3): 281-283. DOI: 10.7506/spkx1002-6630-200903063
Authors:LIN Zhuang-sen  ZHANG Kun  ZHAO Su-qing  ZHENG Jie  HUANG Bao-hua
Affiliation:(Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China)
Abstract:The food poisoning caused by biological toxin has aroused much attention of all governments and the general consumer. The enzyme-linked immunosorbent assay (ELISA) is a method that combines the high efficiency of enzyme in catalyzing with the specific immune reaction between antigen and antibody. This paper reviewed ELISA application that focused on the concentration linear range, sensitivity and recovery in analysis of biotoxins (microbial, animal and plant toxins)in foodstuffs in recently years. The purpose was to promote the application of this technology in food safety examination.
Keywords:ELISA  biotoxin  foodstuff  determination  
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