| 乙醇冷冻提取法纯化磷脂酰胆碱(PC)的工艺研究 |
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| 引用本文： | 罗珍,谢明勇,陈芳,陈军辉,聂少平.乙醇冷冻提取法纯化磷脂酰胆碱(PC)的工艺研究[J].食品研究与开发,2006,27(5):75-77. |
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| 作者姓名： | 罗珍 谢明勇 陈芳 陈军辉 聂少平 |
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| 作者单位： | 南昌大学食品科学教育部重点实验室,江西,南昌,330047 |
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| 摘 要： | 为了得到纯度较高的磷脂酰胆碱,对乙醇冷冻提取法纯化磷脂酰胆碱的工艺进行了研究.采用高效液相色谱法测定磷脂酰胆碱的含量,通过正交实验设计考察了乙醇浓度、料液比和提取时间等因素在不同水平下对磷脂酰胆碱含量的影响,对试验结果进行了方差分析和显著性检验.结果显示乙醇冷冻提取法最佳工艺组合为A2B3C3,即93%乙醇溶液,料液比1：3,冷冻条件下提取8.5 h,在选定的最佳工艺条件下得到的磷脂酰胆碱的含量由原来的33%提高到了75%,可见乙醇冷冻提取法能有效地纯化磷脂酰胆碱.
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| 关 键 词： | 磷脂酰胆碱 乙醇冷冻提取法 纯化 正交设计 |
| 收稿时间： | 2006-03-03 |
| 修稿时间： | 2006年3月3日 |
PURIFICATION OF PHOSPHOTIDYLCHOLINE(PC) WITH ALCOHOL SOLUTION IN FROZEN CONDITION |
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| LUO Zhen,XIE Ming-yong,CHEN Fang,CHEN Jun-hui,NIE Shao-ping.PURIFICATION OF PHOSPHOTIDYLCHOLINE(PC) WITH ALCOHOL SOLUTION IN FROZEN CONDITION[J].Food Research and Developent,2006,27(5):75-77. |
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| Authors: | LUO Zhen XIE Ming-yong CHEN Fang CHEN Jun-hui NIE Shao-ping |
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| Affiliation: | Key Laboratory of Food Science of Ministry of Education, Nanchang University, Nanchang 330047,Jiangxi China |
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| Abstract: | The purification of phosphotidylcholine(PC) with alcohol solution in frozen condition was studied. The influence of alcohol content, the ration of material to solvent and extracting time in different levels was performed by orthogonal plan and content of PC was determined by HPLC. The results showed that the optimal condition for the purification of PC with alcohol solution in frozen condition was 1:3 of 93 % alcohol solution to the sample weight ratio, and 8.5 h, and content of the obtained PC increased up to 75 %, which indicated that high content of PC could be achieved by the method of extracting with alcohol solution in frozen condition. |
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| Keywords: | phosphotidylcholine extracting with alcohol solution in frozen condition purify orthogonal plan |
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