| HPLC法同时测定葡萄和葡萄酒中6种基本花色苷 |
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| 引用本文： | 刘 冰,葛 谦,张 艳,苟春林,赵子丹.HPLC法同时测定葡萄和葡萄酒中6种基本花色苷[J].中国酿造,2017,36(2):162. |
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| 作者姓名： | 刘 冰 葛 谦 张 艳 苟春林 赵子丹 |
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| 作者单位： | 1.宁夏国有林场和林木种苗工作总站，宁夏 银川 750001；2.宁夏农产品质量标准与技术研究所，宁夏 银川 750002 |
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| 基金项目： | 宁夏回族自治区自然基金项目（NZ15108）；宁夏农林科学院科技创新先导资金（NKYJ-15-08） |
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| 摘 要： | 该研究建立了高效液相色谱法同时测定葡萄和葡萄酒中6种基本花色苷的检测方法。样品经无水乙醇/盐酸/水（2∶1∶1, V/V）提取，采用Agilent-ZORBAX SB-C18色谱柱（250 mm×4.6 mm, 5 μm）为固定相，流动相A（乙腈）和B（0.1%磷酸水溶液）梯度洗脱，流速0.8 mL/min，检测波长525 nm。在该色谱条件下，6种花色苷在30 min内得到了很好的分离效果，其含量与峰面积呈现良好线性关系（R＞0.999 3），且回收率为82.2%～93.3%、精密度好（RSD＜5.0%）。该方法方便、快速，能够实现对葡萄和葡萄酒中6种花色苷物质的定性及定量检测。
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| 关 键 词： | 花色苷 高效液相色谱 葡萄 葡萄酒 检测 |
Simultaneous determination of six kinds of basic anthocyanins in grape and wine by HPLC |
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| LIU Bing,GE Qian,ZHANG Yan,GOU Chunlin,ZHAO Zidan.Simultaneous determination of six kinds of basic anthocyanins in grape and wine by HPLC[J].China Brewing,2017,36(2):162. |
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| Authors: | LIU Bing GE Qian ZHANG Yan GOU Chunlin ZHAO Zidan |
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| Affiliation: | 1.Ningxia State Forest Farm and Forest Tree Seedlings Work Station, Yinchuan 750001, China;
2.Quality Standards and Testing Institute of Agricultural Technology, Yinchuan 750002, China |
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| Abstract: | The method for simultaneous determination of six kinds of basic anthocyanins in grape and wine by HPLC was established. The simple was extracted by ethanol/ hydrochloric acid/water (2∶1∶1, V/V), separated by Agilent-ZORBAX SB-C18 chromatographic column (250 mm×4.6 mm, 5 μm), with acetonitrile and 0.1% phosphoric acid solution as mobile phase, flow rate 0.8 ml/min, and detection wavelength 525 nm. Under the chromatographic conditions, the six anthocyanins got good separation effect within 30 min, and the contents showed a good linear relation with the peak area (R＞0.999 3), recovery rate was 82.2%-93.3% and the precision was good (RSD＜5.0%). The method was convenient and fast, which could realize qualitative and quantitative detection of the six anthocyanins in grape and wine. |
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| Keywords: | anthocyanins HPLC grape wine determination |
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