| 培养条件对黑曲霉脂肪酶选择性合成1,3甘油二酯的影响 |
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| 引用本文： | 张富豪,李兰香,何腊平,高 冰,李翠芹,陈翠翠.培养条件对黑曲霉脂肪酶选择性合成1,3甘油二酯的影响[J].中国酿造,2017,36(7):85. |
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| 作者姓名： | 张富豪 李兰香 何腊平 高 冰 李翠芹 陈翠翠 |
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| 作者单位： | 1.贵州大学酿酒与食品工程学院，贵州贵阳550025；2.贵州省农畜产品贮藏与加工重点实验室，贵州贵阳550025；
3.湖北工业大学生物工程与食品学院，湖北武汉430068；4.贵州大学化学与化工学院，贵州贵阳550025 |
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| 基金项目： | 国家自然科学基金（21062004、31660010）；贵州大学研究生创新基金（研理工2016059） |
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| 摘 要： | 从前期筛菌工作中得到的一株能高选择性合成1,3-甘油二酯（1,3-DAG）的产胞内脂肪酶的黑曲霉GZUF36菌株出发，分别研究了培养时间、温度、接种量、装液量和转速对该菌摇瓶发酵的影响，得出合适的培养条件为培养时间72 h，接种量3%，装液量40 mL/250 mL，培养温度28 ℃，转速180 r/min，在此条件下1,3-DAG的得率和选择性分别为23.87%和82.96%。同时也比较了摇瓶发酵和5 L发酵罐发酵的异同，相比于摇瓶发酵，5 L发酵罐发酵的1,3-DAG的得率有所提高，但是发酵罐发酵的时间有所延长。
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| 关 键 词： | 1 3-甘油二酯 脂肪酶 发酵条件 摇瓶 发酵罐 |
Effect of culture conditions on the synthesis of 1,3-DAG by the lipase from Aspergillus niger |
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| ZHANG Fuhao,LI Lanxiang,HE Laping,GAO Bing,LI Cuiqin,CHEN Cuicui.Effect of culture conditions on the synthesis of 1,3-DAG by the lipase from Aspergillus niger[J].China Brewing,2017,36(7):85. |
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| Authors: | ZHANG Fuhao LI Lanxiang HE Laping GAO Bing LI Cuiqin CHEN Cuicui |
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| Affiliation: | 1.School of Liquor and Food Engineering, Guizhou University, Guiyang 550025, China; 2.Key Laboratory of Agricultural and Animal Products Store & Processing of Guizhou Province, Guizhou University, Guiyang 550025, China; 3.College of Bioengineering and Food Science, Hubei University of Technology, Wuhan 430068, China; 4.School of Chemistry and Chemical Engineering, Guizhou University, Guiyang 550025, China |
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| Abstract: | Aspergillus niger GZUF36 with extracellular lipase-producing ability which can highly synthesize 1,3-diglyceride was screened from early screening work. The effects of incubation time, temperature, inoculum, liquid volume, and rotation speed on the shake-flask fermentation were studied. The suitable cultivate conditions were found as followed: incubation time 72 h, inoculum 3%, liquid volume 40 ml/250 ml, temperature 28 ℃, rotation speed 180 r/min. In this condition, the yield and selectivity rate of 1,3-DAG was 23.87% and 82.96%, respectively. The similarities and differences of shake-flask fermentation and 5 L fermentor fermentation were compared, and results showed that the yield of 1,3-DAG was improved by 5 L fermentor fermentation, but the fermentation time extended. |
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| Keywords: | 1 3-diglyceride lipase fermentation condition shake-flask fermentor |
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