| 甘南牦牛肝过氧化氢酶超滤纯化工艺及酶学性质研究 |
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| 引用本文： | 冯 平,李正涛,陈庆安,翟丹云,董延虎.甘南牦牛肝过氧化氢酶超滤纯化工艺及酶学性质研究[J].中国酿造,2017,36(11):130. |
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| 作者姓名： | 冯 平 李正涛 陈庆安 翟丹云 董延虎 |
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| 作者单位： | （甘肃省商业科技研究所有限公司，甘肃 兰州 730020） |
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| 摘 要： | 采用超滤工艺从甘南牦牛肝中分离纯化过氧化氢酶，得出的超滤最佳工艺为超滤膜截留分子质量250 kDa、超滤温度25 ℃、压力0.20 MPa、流速30 m/s、浓缩倍数为3倍。酶学性质研究结果显示，该酶最适温度、pH值分别为35 ℃和7.5，米氏常数（Km）值为53.8 mmol/L。
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| 关 键 词： | 甘南牦牛肝 过氧化氢酶 超滤 纯化 酶学性质 |
| 收稿时间： | 2017-12-29 |
Ultrafiltration purification process and enzymology properties of catalase from Gannan yak liver |
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| FENG Ping,LI Zhengtao,CHEN Qing'an,ZHAI Danyun,DONG Yanhu.Ultrafiltration purification process and enzymology properties of catalase from Gannan yak liver[J].China Brewing,2017,36(11):130. |
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| Authors: | FENG Ping LI Zhengtao CHEN Qing'an ZHAI Danyun DONG Yanhu |
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| Affiliation: | (Gansu Institute of Business and Technology Co., Ltd., Lanzhou 730020, China) |
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| Abstract: | Catalase was separated and purified by ultrafiltration from Gannan yak liver, and the optimal process for ultrafiltration was interception molecular mass ultrafiltration membranet 250 kDa, ultrafiltration temperature 25 ℃, pressure 0.20 MPa, flow rate 30 m/s, concentration multiple three times. The method provided an effective way for industrial scale separation of yak liver catalase. Enzymology properties research results showed that the optimum temperature for the enzyme was 35 ℃, the optimum pH value was 7.5, and the Km value was 53.8 mmol/L. |
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| Keywords: | Gannan yak liver catalase ultrafiltration purification enzymology property |
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