| 豆豉纤溶酶等微生物源纤溶酶的研究与应用进展 |
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| 引用本文： | 沈畅萱,王修俊,黄 珊. 豆豉纤溶酶等微生物源纤溶酶的研究与应用进展[J]. 中国酿造, 2017, 36(8): 6. DOI: 10.11882/j.issn.0254-5071.2017.08.002 |
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| 作者姓名： | 沈畅萱 王修俊 黄 珊 |
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| 作者单位： | 1.贵州大学贵州省发酵工程与生物制药省级重点实验室，贵州贵阳550025；2.贵州大学酿酒与食品工程学院，贵州贵阳550025 |
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| 基金项目： | 贵州省科技计划项目（黔科合支撑[2016]2537） |
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| 摘 要： | 豆豉纤溶酶是从我国传统发酵食品豆豉中分离得到一种具有溶解血纤维蛋白作用的蛋白酶。该文对近五年以豆豉纤溶酶为代表的微生物源纤溶酶的产生菌株筛选诱变与基因克隆表达、发酵工艺优化、酶的分离纯化、纤溶酶功能研究与相关制剂及保健品的开发方面的研究进行综述，对当下国内外对纤溶酶的研究现状进行分析探究，对其应用前景与发展方向进行展望，并对纤溶酶的研究方向提出建议。
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| 关 键 词： | 纤溶酶 菌种筛选 发酵工艺 纯化 |
Research and application progress of fibrinolytic enzyme from Douchi and other microbial source |
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| SHEN Changxuan,WANG Xiujun,HUANG Shan. Research and application progress of fibrinolytic enzyme from Douchi and other microbial source[J]. China Brewing, 2017, 36(8): 6. DOI: 10.11882/j.issn.0254-5071.2017.08.002 |
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| Authors: | SHEN Changxuan WANG Xiujun HUANG Shan |
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| Affiliation: | 1.Guizhou Provincial Key Laboratory of Fermentation Engineering and Biopharmacy, Guizhou University, Guiyang 550025, China;
2.School of Liquor and Food Engineering, Guizhou University, Guiyang 550025, China |
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| Abstract: | Douchi fibrinolytic enzyme, isolated from Chinese traditional food-Douchi, is a protease with fibrin dissolve effect. The research of screening and mutagenesis of strain, gene cloning and expression, optimization of fermentation process, purification of enzymes, function and development of preparations and related preparations and health care products in recent 5 years were reviewed, and the present domestic and overseas condition of fibrinolytic enzyme were analyzed. The application prospect and development direction was expected, and the research directions of fibrinolytic enzyme was suggested. |
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| Keywords: | fibrinolytic enzyme strains screening fermentation process purification |
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