研磨法破碎小球藻细胞工艺优化

选取破碎过程中的关键因素，以油脂得率为评价指标，通过进行单因素试验和正交试验，对研磨法破碎小球藻细胞的工艺条件进行了研究。结果表明，小球藻细胞破碎最佳工艺条件为藻液质量浓度150 g/L，破碎时间2.5 h，球磨机转速400 r/min，藻液与钢珠的质量比为3:4。在此工艺条件下，能达到较好的破碎效果，油脂得率为46.89%。

Optimization of Chlorella cell disruption process by grinding method

The key factors were selected in the process of disruption. Using oil yield as evaluation index, the process conditions of Chlorella cell disruption by grinding method were researched through the single factor and orthogonal experiments. The results indicated that the optimum disruption conditions were optimized as follows: Chlorella liquid concentration 150 g/L, disruption time 2.5 h, rotate speed of ball grinder 400 r/min, the mass ratio of Chlorella liquid and steel ball 3:4. Under the conditions, the disruption effect was better, and the oil yield was 46.89%.