接骨木花青素的纯化工艺及清除DPPH自由基活性的研究

通过乙醇浸提法从接骨木鲜果和干果中提取花青素，通过膜分离技术、正己烷萃取、大孔吸附树脂纯化，从接骨木鲜果和干果中提取得到的花青素冻干粉得率分别为0.43%和1.52%，花青素含量可以达到29.32%。接骨木花青素在质量浓度为200 μg/mL时，DPPH自由基的清除率最高为89.40%，相当于维生素C的96.28%。结果表明，接骨木花青素有一定的抗氧化能力。

Purification process and DPPH free radical scavenging activity of anthocyanidin from Sambucus williamsii

Anthocyanidin was extracted from the fresh and dried fruits of Sambucus williamsii with ethanol extracting method. By membrane separation technology, n-hexane extraction and macroporous adsorption resin purification, the yield of anthocyanidin freeze-dried powder extracted from the fresh and dried fruits of S. williamsii were 0.43% and 1.52%, respectively. The anthocyanidin content was 29.32%. When the content of S. williamsii anthocyanidin was 200 μg/ml, the scavenging rate of anthocyanidin on DPPH free radical was 89.40%, which was equal to that of 96.28% of vitamin C. The results indicated that anthocyanidin from S. williamsii had certain antioxidant capacity.