| 微生物甾醇酯酶的研究进展 |
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| 引用本文： | 任楠楠,王晓辉,迟乃玉,乔 慧,张庆芳. 微生物甾醇酯酶的研究进展[J]. 中国酿造, 2017, 36(6): 9. DOI: 10.11882/j.issn.0254-5071.2017.06.002 |
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| 作者姓名： | 任楠楠 王晓辉 迟乃玉 乔 慧 张庆芳 |
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| 作者单位： | 1.大连大学生命科学与技术学院，辽宁大连116622；2.辽宁省海洋微生物工程技术研究中心，辽宁大连116622 |
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| 基金项目： | 国家高技术研究发展计划‘863计划’项目（2007AA021306） |
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| 摘 要： | 甾醇酯酶（EC 3.1.1.13）作为一种高活性的生物催化剂，在医疗检测、食品、造纸工业及环保等领域具有极其广泛的应用价值。该文综述了甾醇酯酶的微生物来源、分离纯化、克隆表达、三维结构与应用方面的研究进展，以期为甾醇酯酶的研究开发及产业化应用奠定基础。
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| 关 键 词： | 甾醇酯酶 微生物 克隆表达 分离纯化 应用 |
Research advance in sterol esterase from microorganisms |
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| .College of Life Science and Biotechnology,Dalian University,Dalian,China. Research advance in sterol esterase from microorganisms[J]. China Brewing, 2017, 36(6): 9. DOI: 10.11882/j.issn.0254-5071.2017.06.002 |
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| Authors: | .College of Life Science Biotechnology Dalian University Dalian China |
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| Affiliation: | 1.College of Life Science and Biotechnology, Dalian University, Dalian 116622, China;
2.Liaoning Technology of Marine Microbiological Engineering Research Center, Dalian 116622, China |
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| Abstract: | Sterol esterase (EC 3.1.1.13), as a high activity biocatalyst, has extremely broad application value in medical detection, food, paper industry and environmental protection, etc. The research advance of microorganism's origin, separation and purification, cloning and expression, three-dimensional structure and application of sterol esterase were summarized, which might lay foundation for research, development and industrialization application of sterol esterase. |
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| Keywords: | sterol esterase microorganism cloning and expression separation and purification application |
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