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富含谷氨酸酿酒酵母的筛选及发酵培养基优化
引用本文:刘立聪,胡依雯,王 志,代 俊,李 欣,姚 娟,郑国斌,吕正波,陈 雄.富含谷氨酸酿酒酵母的筛选及发酵培养基优化[J].中国酿造,2017,36(6):116.
作者姓名:刘立聪  胡依雯  王 志  代 俊  李 欣  姚 娟  郑国斌  吕正波  陈 雄
作者单位:1.湖北工业大学生物工程与食品学院 发酵工程教育部重点试验室工业发酵湖北省协同创新中心,湖北武汉430068; 2.安琪酵母股份有限公司湖北省酵母功能重点试验室,湖北宜昌443003,3.安琪酵母(德宏)有限公司,云南德宏678400
摘    要:从农家自酿葡萄酒中筛选出一株富含谷氨酸酿酒酵母菌(Saccharomyces cerevisiae)F-5,其26S rDNA核苷酸序列与S. cerevisiae TY12的26S rDNA核苷酸序列同源性为100%。以胞内谷氨酸含量为目标,采用响应面法对其发酵培养基进行了优化,建立糖蜜、工业蛋白胨和KH2PO4的二次回归模型,确定培养基最佳配方为:糖蜜(含30%蔗糖)100 mL/L、酵母浸粉10 g/L、工业蛋白胨20 g/L、MgSO4·7H2O 1 g/L、KH2PO4 0.5 g/L、FeSO4·7H2O 2 g/L。在此优化培养基中发酵培养24 h,胞内游离谷氨酸达到了3.29%,比优化前提高了87.8%。

关 键 词:酿酒酵母  胞内游离谷氨酸  发酵培养基  响应面法  

Screening of Saccharomyces cerevisiae with high glutamic acid content and optimization of fermentation medium
LIU Licong,HU Yiwen,WANG Zhi,DAI Jun,LI Xin,YAO Juan,ZHENG Guobin,LV Zhengbo,CHEN Xiong.Screening of Saccharomyces cerevisiae with high glutamic acid content and optimization of fermentation medium[J].China Brewing,2017,36(6):116.
Authors:LIU Licong  HU Yiwen  WANG Zhi  DAI Jun  LI Xin  YAO Juan  ZHENG Guobin  LV Zhengbo  CHEN Xiong
Affiliation:1.Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Key Laboratory Fermentation Engineering (Ministry of Education), College of Food and Bioengineering, Hubei University of Technology, Wuhan 430068, China; 2.Hubei Province Key
Laboratory of Yeast Function, Angel Yeast Co., Ltd., Yichang 443003, China; 3.Angel Yeast (De Hong) Co., Ltd., Dehong 678400, China
Abstract:A Saccharomyces cerevisiae F-5 with high glutamic acid content was screened from home-made wine. The homology of its 26S rDNA sequence with the 26S rDNA sequence of S. cerevisiae TY12 was 100%. Using the intracellular glutamate content as evaluation indexes, the fermentation medium of the strain F-5 was optimized by response surface methodology, and quadratic regression model of molasses, industrial peptone and KH2PO4 was established. The optimum formula of fermentation medium were determined as follows: molasses (30% sucrose) 100 ml/L, yeast extract powder 10 g/L, industrial peptone 20 g/L, MgSO4·7H2O 1 g/L, K2HPO4 0.5 g/L, and FeSO4·7H2O 2 g/L. The content of intracellular free glutamate was up to 3.29% under the optimum medium at 24 h, which was 87.8% higher than that of before optimization.
Keywords:Saccharomyces cerevisiae  intracellular free glutamate  fermentation medium  response surface methodology  
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