林可霉素工业生产发酵工艺优化

为了提高林可霉素工业生产发酵效价，该研究对林可霉素发酵工艺进行了优化。结果表明，林可霉素最佳发酵条件为二级种子罐发酵培养基中淀粉和黄豆饼粉添加量分别为0.97%和1.43%、延长二级种子移种菌龄至48 h及四级发酵罐基础料计料体积为8 m3。在此优化发酵工艺条件下，林可霉素发酵四级放罐效价高达7 505 U/mL，比原始工艺四级放罐效价提高了16%。初步优化了林可霉素工业生产发酵工艺，提高了林可霉素发酵效能和产量。

Optimization of fermentation process of lincomycin industrial production

In order to increase the fermentation potency of lincomycin industrial production, the fermentation process of lincomycin was optimized. The results showed that the optimum fermentation conditions of lincomycin were as follow: the concentrations of starch and soybean cake powder in the fermentation medium of two-stage seeds fermenter were 0.97% and 1.43%, respectively, the transplanting cell age of two-stage seeds was extended to 48 h and the volume of the base material of four-stage fermentation tank was 8 m3. Under the optimum fermentation conditions, the fermentation potency of lincomycin after four-stage fermentation was up to 7 505 U/ml, which was 16% higher than that of the original fermentation process. The fermentation process of lincomycin industrial production was optimized preliminarily, and the fermentation efficiency and yield of lincomycin were improved.