固相萃取-高效液相色谱串联质谱法测定植物油中6种酚类环境激素

建立了一种简单、快速测定植物油中6种酚类环境激素（双酚A、双酚B、双酚F、4-壬基酚、4-n-壬基酚、辛基酚）的固相萃取净化-液相色谱串联质谱的分析方法。样品加入同位素内标后，经正己烷溶解，Carb/NH2固相萃取柱净化，高效液相色谱分离，串联四极杆电喷雾离子源负模式及多反应监测模式检测。双酚A等6种环境激素在0.40~200 μg/L范围内线性关系良好，相关系数均＞0.994；方法的检出限为0.4～0.8 μg/kg，定量限为1.2～2.4 μg/kg；平均回收率为87.9%～98.0%，相对标准偏差为4.37%～11.00%。该方法具有净化效果好、定量准确、灵敏快速的特点，适用于植物油中双酚A等6种环境激素的定量分析检测。

Determination of six phenol environmental hormones in plant oil by solid-phase extraction coupled with HPLC-MS/MS

A simple and quick analysis method was established for the determination of six phenol environmental hormones (bisphenol A, bisphenol B, bisphenol F, 4-nonylphwnol, 4-n-nonylphenol, octylphenol) in plant oil by HPLC-MS/MS and solid phase extraction (SPE). After addition of the isotope internal standard, the samples were dispersed with hexane and cleaned by Carb/NH2 solid phase extraction column, and then analyzed by HPLC-MS/MS in the multiple reaction monitoring (MRM) mode. Under the optimal experimental conditions, the calibration curves of six phenol environmental hormones were linear in the range of 0.40-50.0 μg/L with correlation coefficients more than 0.994. The limits of detection and the limits of qualification of the method were 0.4-0.8 μg/kg and 1.2-2.4 μg/kg, respectively. The mean recovery rate of six phenol environmental hormones were 87.9%-98.0% combining with the relative standard deviation ranging from 4.37%-11.00%. The method was suitable for the identification and quantification of six phenol environmental hormones in plant oil due to its simplicity and accuracy.