| 海洋肌酐水解酶菌株的筛选鉴定及其酶学性质研究 |
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| 引用本文： | 石 群,张庆芳,杨丽娜,王晓辉,窦少华,迟乃玉. 海洋肌酐水解酶菌株的筛选鉴定及其酶学性质研究[J]. 中国酿造, 2017, 36(3): 19. DOI: 10.11882/j.issn.0254-5071.2017.03.005 |
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| 作者姓名： | 石 群 张庆芳 杨丽娜 王晓辉 窦少华 迟乃玉 |
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| 作者单位： | 1.大连大学生命科学与技术学院，辽宁大连116622；2.辽宁省海洋微生物工程技术研究中心，辽宁大连116622 |
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| 基金项目： | 国家高技术研究发展计划“863”计划项目（2007AA021306）；辽宁省自然科学基金（NO.2050775） |
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| 摘 要： | 从渤海海泥中筛选出一株肌酐水解酶高产菌株S-09，经形态学、生理生化特征并结合16S rDNA系统发育分析，鉴定菌株为微小杆菌属(Exiguobacterium sp.)。对所产肌酐水解酶进行酶学性质研究表明，该酶的最适作用温度为30 ℃，热稳定性较差；最适作用pH值为7.5，碱性条件下，pH值稳定性较好；Co2+、Mn2+对酶的激活作用较强，Ag+、Hg2+对酶有显著的抑制作用。
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| 关 键 词： | 海洋 肌酐水解酶 菌株筛选 鉴定 酶学性质 |
Screening and identification of creatininase-producing strain from marine and its enzymatic properties |
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| SHI Qun,ZHANG Qingfang,YANG Lina,WANG Xiaohui,DOU Shaohua,CHI Naiyu. Screening and identification of creatininase-producing strain from marine and its enzymatic properties[J]. China Brewing, 2017, 36(3): 19. DOI: 10.11882/j.issn.0254-5071.2017.03.005 |
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| Authors: | SHI Qun ZHANG Qingfang YANG Lina WANG Xiaohui DOU Shaohua CHI Naiyu |
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| Affiliation: | 1.School of Life Science and Biotechnology, Dalian University, Dalian 116622, China;
2.Liaoning Marine Microbial Engineering and Technology Center, Dalian 116622, China |
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| Abstract: | A high creatininase-producing strain S-09 was screened from the Bohai Sea mud, and identified as Exiguobacterium sp. by morphology, physiological and biochemical characteristics and 16S rDNA phylogenetic analysis. The research of enzymatic properties of creatininase showed that the optimal temperature was 30 ℃, the thermostability was weaker; the optimal pH was 7.5, pH stability was better in alkaline condition; the activation of Co2+ and Mn2+ on creatininase was stronger, the Ag+ and Hg2+ had significant inhibitory effect on creatininase. |
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| Keywords: | marine creatininase strain screening identification enzymatic characteristics |
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