红薯淀粉废水中乳酸菌的筛选及其对食源性致病菌的抑制作用

从采集的红薯淀粉废水中分离出3株乳酸菌SPDJ3、SPEJ7 和SPEJ9。通过培养特征、形态特征，生理生化特征及基于16S rDNA序列的系统发育分析的检测，菌株SPDJ3和SPEJ7被鉴定为植物乳杆菌（Lactobacillus plantarum），菌株SPEJ9被鉴定为副干酪乳杆菌（Lactobacillus paracasei）。3株乳酸菌发酵上清液对供试8株食源性致病菌均有抑菌作用，菌株SPDJ3对福氏志贺氏菌（Shigella flexneri）抑制效果较强，抑菌圈直径为（30.37±0.15） mm，菌株SPEJ7和SPEJ9对蜡样芽胞杆菌（Bacillus cereus）抑制效果较强，抑菌圈直径分别为（29.43±0.21） mm和（28.27±0.25） mm。

Screening of lactic acid bacteria from sweet potato starch processing wastewater and its antibacterial activity against food-borne pathogenic bacteria

Three strains of lactic acid bacteria SPDJ3, SPEJ7 and SPEJ9 were isolated from the collected sweet potato starch wastewater. Based on the cultural, morphological, physiological and biochemical characteristics and phylogenetic analysis of 16S rDNA sequence, strains SPDJ3 and SPEJ7 were identified as Lactobacillus plantarum, strain SPEJ9 was identified as Lactobacillus paracasei. The fermentation supernatant liquid of the 3 strains showed antibacterial effect on 8 tested strains of food-borne pathogenic bacteria, strain SPDJ3 had strong antibacterial effect on Shigella flexneri, the diameter of the inhibition zone was (30.37±0.15) mm. However, the bacteriostatic effects of strain SPEJ7 and SPEJ9 on Bacillus cereus were stronger, and the diameters of the inhibition zones were (29.43±0.21) mm and (28.27±0.25) mm, respectively.