固相萃取-气相色谱/质谱法同时测定植物油中胆固醇和维生素E

建立了固相萃取（SPE）净化结合气相色谱-质谱联用（GC-MS）同时检测植物油中胆固醇和维生素E四种异构体（α-生育酚、β-生育酚、γ-生育酚和δ-生育酚）的分析方法。样品采用氨基固相萃取小柱提取净化，洗脱液经DB-5MS色谱柱分离，GC-MS选择离子监测模式（SIM）测定。实验表明，胆固醇和维生素E四种异构体在各自浓度范围内线性关系良好。方法的回收率为83.9%～108.0%，相对标准偏差（RSD）为1.3%～9.3%，胆固醇和α-生育酚、β-生育酚、γ-生育酚和δ-生育酚的检出限分别为0.2 mg/kg、1.0 mg/kg、2.0 mg/kg、2.0 mg/kg、0.8 mg/kg。结果表明，该方法结果可靠，可用于日常检测。

Simultaneous determination of cholesterol and vitamin E in vegetable oil by solid phase extraction and gas chromatography-mass spectrometry

An analysis method was established for the simultaneous detection of cholesterol and four kinds of vitamin E isomers (alpha tocopherol, beta tocopherol, gamma tocopherol and delta tocopherol) in vegetable oil using solid phase extraction (SPE) and gas chromatography-mass spectrometry (GC-MS). Samples were concentrated and purified by NH2 SPE cartridges. The eluent was separated by DB-5MS capillary chromatographic column and detected by selected ion monitoring (SIM) mode of GC-MS. Experiments results showed that the cholesterol and four kinds of vitamin E isomers were linear in their respective concentration range. The recovery rate of the method for target compounds was 83.9%-108.0%. The relative standard deviation (RSD) was 1.3%-9.3%. Limits of detection of cholesterol and alpha tocopherol, beta tocopherol, gamma tocopherol and delta tocopherol were 0.2 mg/kg, 1.0 mg/kg, 2.0 mg/kg, 2.0 mg/kg, and 0.8 mg/kg, respectively. The results showed that the analysis method was reliable and can be used for daily inspection.