气相色谱-质谱法测定酵素产品中短链脂肪酸

建立了用气相色谱-质谱法（GC-MS）测定酵素样品中5种短链脂肪酸（SCFA）的定性定量方法。结果表明，该方法可以有效分离测定酵素样品中5种SCFA，该方法检出限低（0.030～0.100 mg/kg），加标回收率80.1%～110.6%，相对标准偏差（RSD）1.36%～10.0%。用该方法分析37种酵素样品中SCFA，结果表明，86.5%的酵素产品检出乙酸（1.02～36.1 g/kg），仅有一种酵素产品检出丁酸（含量为0.056 g/kg），其余均未检出。该方法适用于酵素产品中短链脂肪酸的检测。

Determination of short-chain fatty acids in ferments by gas chromatography-mass spectrometry

The qualitative and quantitative analysis methods of 5 kinds of short-chain fatty acids (SCFA) in ferments samples were established by gas chromatography-mass spectrometry (GC-MS). The results showed that the method could effectively separate the five kinds of SCFA in ferments samples, and had low limits of detection (0.030-0.100 mg/kg), adding standard recovery rate range 80.1%-110.6%, and relative standard deviation (RSD) 1.36%-10.0%. The determination results of 37 kinds of ferments samples analysis by the method indicated that acetic acid was detected in 86.5% ferments samples (1.02-36.1 g/kg), butyric acid was detected in only one sample (0.056 g/kg), and other components were not detected. Therefore, the method could be used for determination of five SCFAs in ferments products.