过滤法分离纯化透明质酸

针对透明质酸的分离纯化，提出了用过滤法分离纯化透明质酸的工艺. 首先以20 g/L的溶解浓度将透明质酸粗品溶于去离子水中，并加入硅藻土作为吸附剂，调节溶液pH至4.6~4.8；然后采用硅藻土过滤、滤板过滤、超滤，用乙醇结晶后可得葡萄糖醛酸含量高达44.2%的透明质酸. 该工艺已成功应用于工业生产，所得产品的葡萄糖醛酸含量达47.3%，蛋白质含量为0.01%，相对分子量可达110万，收率高达90.5%.

Separating and Purifying Hyaluronic Acid by Filtration Method

A filtration method was developed to separate and purify hyaluronic acid (HA) from fermentation broth. Crude HA was dissolved in distilled water and reached its concentration of 20 g/L. Silicon earth as sorbent was added into the HA solution. pH of the solution was adjusted between 4.6~4.8. Then, the solution was filtrated with silicon earth, filter B-1 and ultrafiltration membrane. Finally the HA solution was crystallized by ethanol, thus, farinose HA containing glucuronic acid 44.2% was prepared. This method was successfully applied to the industry. The relative molecular weight of factory product was up to 1.1 million. The factory product contained 47.3% glucuronic acid and 0.01% protein. The yield of HA reach to 90.5% in the factory. The cost of the production was reduced to 64.3% by the filtration method in this work.