麦草酸水解及水解液木糖发酵的研究

采用甲酸/盐酸水解体系水解麦草,发现 65℃ 水解 0.5 h 可实现麦草中半纤维素的充分水解。选用热带假丝酵母发酵麦草甲酸水解液制取木糖醇。分别研究了在不同浓度甲酸及甲酸根条件下D-木糖的发酵效果,发现在 2 g/L 的甲酸及 5 g/L 的甲酸根条件下,D-木糖实现发酵并得到最大产率的木糖醇,高浓度的甲酸及甲酸根都会抑制D-木糖的发酵。采用D311型阴离子交换树脂脱除甲酸根,实现了麦草水解液的发酵,木糖醇最高得率为 16.88%(木糖醇/木糖)。

Study on Acid Hydrolysis of Wheat Straw and Fermentation of Xylose in Hydrolysate to Xylitol

Hemicellulose could be hydrolyzed adequately when wheat straw was retained in the system of formic acid/hydrochloric acid for 0.5 h under 65℃. Straw hydrolysate was fermented by Candida tropicalis to produce xylitol. The effects of different concentration of formic acid and formate on the fermentation of D-xylose were studied. D-xylose could be fermented to produce xylitol availably with a concentration of 2 g/L formic acid or 5 g/L formate. A high concentration of formic acid or formate restrained the fermentation of D-xylose. Straw formic acid hydrolysate could be fermented to produce xylitol after the formate was removed by ion exchange resins of D311. All xylose was consumed within 72 h with a xylitol yield of 16.88%(xylitol/xylose).