Metabolism of sterols of varying ring unsaturation and methylation byCaenorhabditis elegans |
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| Authors: | Ruben Lozano Thomas A Salt David J Chitwood William R Lusby Malcolm J Thompson |
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| Affiliation: | (1) Insect and Nematode Hormone Laboratory, Agricultural Research Service, USDA, Beltsville, Maryland;(2) Department of Botany, University of Maryland, College Park, Maryland;(3) Insect and Nematode Hormone Laboratory, Bldg. 467, BARC-East, 20705 Beltsville, MD |
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| Abstract: | The metabolism of three dietary 4,4-desmethylsterols and two 4α-methylsterols was investigated in the free-living nematodeCaenorhabditis elegans. Dietary cholestanol was converted mostly to lathosterol. Dietary lathosterol, 7-dehydrocholesterol, 4α-methylcholest-7-enol
and 4α-methylcholest-8(14)-enol each remained largely unchanged. An absolute requirement for a substantial quantity of 7-dehydrocholesterol
inC. elegans did not exist.C. elegans was unable to remove a 4α-methyl group or introduce a double bond at C-5 and also demonstrated the lack of a Δ7-reductase. Its nutritional sterol requirement was satisfied by cholestanol, lathosterol or 7-dehydrocholesterol; growth was
comparable to that obtained previously in media containing Δ5-sterols. However, the two 4α-methylsterols appeared to be unsatisfactory sterol nutrients. The possible physiological importance
of 4α-methylsterols is discussed briefly. |
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